Zebrafish Jak2a plays a crucial role in definitive hematopoiesis and blood vessel formation

Jin Jea Sung, Jinseon Jeon, Jong Joo Lee, Chul Geun Kim

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We examined the role of zebrafish (Danio rerio) Jak2a, a homolog of mammalian Jak2, in the developing embryo by injecting in vitro synthesized Jak2a shRNA into zebrafish zygotes. Blood circulation was suppressed in Jak2a shRNA-injected embryos from 24 hours post fertilization (hpf) and all embryos died with enlarged pericardium, shortened body lengths, and defects in some vasculature within 8 days post fertilization. O-dianisidine staining of red blood cells revealed normal blood island formation with no circulating red blood cells. As in Jak2-/- transgenic mice, expression of definitive Ba1 globin was significantly reduced in Jak2a knockdown embryos at 36 hpf, whereas expression of other hematopoietic markers, primitive be1 globin, gata-1, and scl, were unaffected. More importantly, blood vessel formation was disturbed in Jak2a knockdown embryos as revealed by alkaline phosphatase staining at 72 hpf. Thus, our data indicate that zebrafish Jak2a is important in both definitive hematopoiesis and blood vessel formation.

Original languageEnglish
Pages (from-to)629-633
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume378
Issue number3
DOIs
StatePublished - 2009 Jan 16

Keywords

  • Blood vessel formation
  • Gene knockdown
  • Hematopoiesis
  • Jak2a
  • Zebrafish
  • shRNA

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