Vasopressin increases Na-K-2Cl cotransporter expression in thick ascending limb of Henle's loop

Gheun-Ho Kim, Carolyn A. Ecelbarger, Carter Mitchell, Randall K. Packer, James B. Wade, Mark A. Knepper

Research output: Contribution to journalArticle

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Abstract

To investigate whether the enhancement of thick ascending limb (TAL) NaCl transport in response to long-term increases in circulating vasopressin concentration is associated with increased expression levels of the apical Na-K-2Cl cotransporter in the rat TAL, we have carried out immunoblotting and immunofluorescence studies using affinity-purified, peptide-directed antibodies. Semiquantitative immunoblotting studies demonstrated a marked increase (193% of controls) in Na-K-2Cl cotransporter band density in response to restriction of water intake to 15 ml/day for 7 days. In contrast, the expression levels of two other apical proteins of the TAL (the type 3 Na/H exchanger and Tamm-Horsfall protein) were unchanged in the outer medulla. A 7-day subcutaneous infusion of the V2 receptor-selective vasopressin analog, 1-desamino-[8-D-arginine]vasopressin (DDAVP), to Brattleboro rats also markedly increased Na-K-2Cl cotransporter expression in the outer medulla (183% of controls). Immunofluorescence localization in outer medullary tissue sections confirmed the increase in Na-K-2Cl cotransporter expression in response to DDAVP. We conclude that vasopressin strongly upregulates the expression of the Na-K-2Cl cotransporter of the TAL and that it is likely to play an important role in the long-term regulation of the countercurrent multiplication system.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Physiology
Volume276
Issue number1 45-1
StatePublished - 1999 Jan 1

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Loop of Henle
Vasopressins
Extremities
Immunoblotting
Fluorescent Antibody Technique
Uromodulin
Brattleboro Rats
Subcutaneous Infusions
Deamino Arginine Vasopressin
Vasopressin Receptors
Sodium-Hydrogen Antiporter
Arginine Vasopressin
Drinking
Up-Regulation
Peptides
Antibodies
Proteins

Keywords

  • Antidiuretic hormone
  • Countercurrent multiplication
  • Sodium chloride transport

Cite this

Kim, G-H., Ecelbarger, C. A., Mitchell, C., Packer, R. K., Wade, J. B., & Knepper, M. A. (1999). Vasopressin increases Na-K-2Cl cotransporter expression in thick ascending limb of Henle's loop. American Journal of Physiology - Renal Physiology, 276(1 45-1).
Kim, Gheun-Ho ; Ecelbarger, Carolyn A. ; Mitchell, Carter ; Packer, Randall K. ; Wade, James B. ; Knepper, Mark A. / Vasopressin increases Na-K-2Cl cotransporter expression in thick ascending limb of Henle's loop. In: American Journal of Physiology - Renal Physiology. 1999 ; Vol. 276, No. 1 45-1.
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Vasopressin increases Na-K-2Cl cotransporter expression in thick ascending limb of Henle's loop. / Kim, Gheun-Ho; Ecelbarger, Carolyn A.; Mitchell, Carter; Packer, Randall K.; Wade, James B.; Knepper, Mark A.

In: American Journal of Physiology - Renal Physiology, Vol. 276, No. 1 45-1, 01.01.1999.

Research output: Contribution to journalArticle

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T1 - Vasopressin increases Na-K-2Cl cotransporter expression in thick ascending limb of Henle's loop

AU - Kim, Gheun-Ho

AU - Ecelbarger, Carolyn A.

AU - Mitchell, Carter

AU - Packer, Randall K.

AU - Wade, James B.

AU - Knepper, Mark A.

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AB - To investigate whether the enhancement of thick ascending limb (TAL) NaCl transport in response to long-term increases in circulating vasopressin concentration is associated with increased expression levels of the apical Na-K-2Cl cotransporter in the rat TAL, we have carried out immunoblotting and immunofluorescence studies using affinity-purified, peptide-directed antibodies. Semiquantitative immunoblotting studies demonstrated a marked increase (193% of controls) in Na-K-2Cl cotransporter band density in response to restriction of water intake to 15 ml/day for 7 days. In contrast, the expression levels of two other apical proteins of the TAL (the type 3 Na/H exchanger and Tamm-Horsfall protein) were unchanged in the outer medulla. A 7-day subcutaneous infusion of the V2 receptor-selective vasopressin analog, 1-desamino-[8-D-arginine]vasopressin (DDAVP), to Brattleboro rats also markedly increased Na-K-2Cl cotransporter expression in the outer medulla (183% of controls). Immunofluorescence localization in outer medullary tissue sections confirmed the increase in Na-K-2Cl cotransporter expression in response to DDAVP. We conclude that vasopressin strongly upregulates the expression of the Na-K-2Cl cotransporter of the TAL and that it is likely to play an important role in the long-term regulation of the countercurrent multiplication system.

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