Upregulation of interleukin-1β production by 1,25-Dihydroxyvitamin D3 in activated human macrophages

Bit Na Ra Lee, Tae Hwan Kim, Jae Bum Jun, Dae Hyun Yoo, Jin Hyun Woo, Sung Jae Choi, Young Ho Lee, Gwan Gyu Song, Yongjin Kim, Ji Young Lee, Jeongwon Sohn, Jong Dae Ji

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) plays important roles in the immune system. In contrast to its well known function in the adaptive immune system, much less is known about the immunoregulatory effects of 1,25(OH)2D3 in the innate immune system, especially on activated human macrophages. Here we found that 1,25(OH)2D3 strongly stimulated the production of interleukin-1β (IL-1β) in PMA-differentiated U937 cells and human monocyte-derived macrophages treated with lipopolysaccharide (LPS) or PMA. In this study, Erk1/2 appeared to mediate 1,25(OH)2D3-induced expression of IL-1β. Parallel to the increased production of IL-1β, 1,25(OH)2D3 increased the expression and phosphorylation of the CCAAT enhancer-binding protein β (C/EBPβ), which is one of the key transcriptional regulatory factors for IL-1β transcription. These results suggest that 1,25(OH)2D3 may function as a proinflammatory molecule in inflammatory macrophages.

Original languageEnglish
Pages (from-to)2193-2201
Number of pages9
JournalMolecular Biology Reports
Volume38
Issue number3
DOIs
StatePublished - 2011 Mar 1

Keywords

  • 1,25-Dihydroxyvitamin D
  • IL-1β
  • Inflammation
  • Macrophages

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    Lee, B. N. R., Kim, T. H., Jun, J. B., Yoo, D. H., Woo, J. H., Choi, S. J., Lee, Y. H., Song, G. G., Kim, Y., Lee, J. Y., Sohn, J., & Ji, J. D. (2011). Upregulation of interleukin-1β production by 1,25-Dihydroxyvitamin D3 in activated human macrophages. Molecular Biology Reports, 38(3), 2193-2201. https://doi.org/10.1007/s11033-010-0348-z