Unique isozymes of superoxide dismutase in Streptomyces griseus

Hong Duk Youn, Hwan Youn, Jin Won Lee, Yang In Yim, Jeong Kug Lee, Yung Chil Hah, Sa Ouk Kang

Research output: Contribution to journalArticle

124 Scopus citations

Abstract

Two unique isozymes of superoxide dismutase (EC 1.15.1.1) were purified to apparent homogeneity from Streptomyces griseus by a purification procedure consisting of ammonium sulfate precipitation and chromatographies on DEAE Sephacel, Sephacryl S-200, and DEAE 5PW. Superoxide dismutase I was composed of four identical subunits of 13.0 kDa. The absorption spectrum of superoxide dismutase I exhibited absorption bands at 276 and 378 nm and a broad shoulder at 530 nm. The g values of electron paramagnetic resonance spectrum of superoxide dismutase I were g1 = 2.304, g2 = 2.248, and g3 = 2.012 and the resonance centered at g3 = 2.012 was split into triplet, indicating nickel- containing superoxide dismutase. Superoxide dismutase I contained 0.89 g- atom of nickel per mole of 13.0-kDa subunit. Superoxide dismutase II was composed of four identical subunits of 22.0 kDa. The absorption spectrum of superoxide dismutase II showed the featureless absorption band in the range of 300500 nm. The g values of electron paramagnetic resonance spectrum of superoxide dismutase II were g(z) = 4.762, g(x) = 4.072, and g(y) = 3.742, indicating iron-containing superoxide dismutase. Superoxide dismutase II uniquely contains 0.40 g-atom of iron per mole of monomer as well as 0.43 g- atom of zinc per mole of monomer. The immunological cross-reactivity between two isozymes was not found. Nickel-containing superoxide dismutase was widely distributed within the genus Streptomyces; however, iron- and zinc-containing superoxide dismutase was not found in S. albus and S. longisporoflavus, on the basis of the immunological cross-reactivity.

Original languageEnglish
Pages (from-to)341-348
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume334
Issue number2
DOIs
StatePublished - 1996 Oct 15

Keywords

  • EPR spectrum
  • Streptomyces griseus
  • iron- and zinc-superoxide dismutase
  • nickel-superoxide dismutase

Fingerprint Dive into the research topics of 'Unique isozymes of superoxide dismutase in Streptomyces griseus'. Together they form a unique fingerprint.

  • Cite this

    Youn, H. D., Youn, H., Lee, J. W., Yim, Y. I., Lee, J. K., Hah, Y. C., & Kang, S. O. (1996). Unique isozymes of superoxide dismutase in Streptomyces griseus. Archives of Biochemistry and Biophysics, 334(2), 341-348. https://doi.org/10.1006/abbi.1996.0463