Two thioredoxin reductases, trxr-1 and trxr-2, have differential physiological roles in caenorhabditis elegans

Weixun Li, Jaya Bandyopadhyay, Hyun Sook Hwaang, Byung Jae Park, Jeong Hoon Cho, Jin Il Lee, Joohong Ahnn, Sun Kyung Lee

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Thioredoxin reductase (TrxR) is a member of the pyridine nucleotide-disulfide reductase family, which mainly functions in the thioredoxin system. TrxR is found in all living organisms and exists in two major ubiquitous isoenzymes in higher eukaryotic cells; One is cytosolic and the other mitochondrial. Mitochondrial TrxR functions to protect mitochondria from oxidative stress, where reactive oxidative species are mainly generated, while cytosolic TrxR plays a role to maintain optimal oxido-reductive status in cytosol. In this study, we report differential physiological functions of these two TrxRs in C. elegans. trxr-1, the cytosolic TrxR, is highly expressed in pharynx, vulva and intestine, whereas trxr-2, the mitochondrial TrxR, is mainly expressed in pharyngeal and body wall muscles. Deficiency of the non-selenoprotein trxr-2 caused defects in longevity and delayed development under stress conditions, while deletion mutation of the selenoprotein trxr-1 resulted in interference in acidification of lysosomal compartment in intestine. Interestingly, the acidification defect of trxr-1(jh143) deletion mutant was rescued, not only by selenocystein-containing wild type TRXR-1, but also cysteine- substituted mutant TRXR-1. Both trxr-1 and trxr-2 were up-regulated when worms were challenged by environmental stress such as heat shock. These results suggest that trxr-1 and trxr-2 function differently at organismal level presumably by their differential sub-cellular localization in C. elegans.

Original languageEnglish
Pages (from-to)209-218
Number of pages10
JournalMolecules and Cells
Volume34
Issue number2
DOIs
StatePublished - 2012 Aug 1

Fingerprint

Thioredoxin Reductase 1
Thioredoxin-Disulfide Reductase
Caenorhabditis elegans
Thioredoxin Reductase 2
Intestines
Selenoproteins
Thioredoxins
Vulva
Sequence Deletion
Eukaryotic Cells
Pharynx
Disulfides
Cytosol
Isoenzymes
Cysteine
Shock
Mitochondria
Oxidoreductases
Oxidative Stress
Nucleotides

Keywords

  • C. Elegans
  • Longevity
  • Oxidative stress
  • Thioredoxin reductase
  • V-ATPase

Cite this

Li, W., Bandyopadhyay, J., Hwaang, H. S., Park, B. J., Cho, J. H., Lee, J. I., ... Lee, S. K. (2012). Two thioredoxin reductases, trxr-1 and trxr-2, have differential physiological roles in caenorhabditis elegans. Molecules and Cells, 34(2), 209-218. https://doi.org/10.1007/s10059-012-0155-6
Li, Weixun ; Bandyopadhyay, Jaya ; Hwaang, Hyun Sook ; Park, Byung Jae ; Cho, Jeong Hoon ; Lee, Jin Il ; Ahnn, Joohong ; Lee, Sun Kyung. / Two thioredoxin reductases, trxr-1 and trxr-2, have differential physiological roles in caenorhabditis elegans. In: Molecules and Cells. 2012 ; Vol. 34, No. 2. pp. 209-218.
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Two thioredoxin reductases, trxr-1 and trxr-2, have differential physiological roles in caenorhabditis elegans. / Li, Weixun; Bandyopadhyay, Jaya; Hwaang, Hyun Sook; Park, Byung Jae; Cho, Jeong Hoon; Lee, Jin Il; Ahnn, Joohong; Lee, Sun Kyung.

In: Molecules and Cells, Vol. 34, No. 2, 01.08.2012, p. 209-218.

Research output: Contribution to journalArticle

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AU - Li, Weixun

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