Trichomonas vaginalis-induced neutrophil apoptosis causes anti-inflammatory cytokine production by human monocyte-derived macrophages

M. H. Ahn, H. O. Song, J. S. Ryu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Neutrophils are the predominant inflammatory cells found in the vaginal discharge of patients with a Trichomonas vaginalis infection. Neutrophils have a shorter life span than other leucocytes. Our previous study indicated that live T. vaginalis alters Mcl-1 expression and caspase-3 activation, thereby inducing apoptosis of human neutrophils. However, it was previously unknown that the apoptotic neutrophils brought about by T. vaginalis can influence vaginal inflammation. Thus, human monocyte-derived macrophages (HMDM) were incubated with T. vaginalis-induced apoptotic neutrophils. Cytokine production and phagocytosis by HMDM were evaluated by ELISA and myeloperoxidase stain, respectively. HMDM showed increased anti-inflammatory cytokine production (IL-10) and decreased levels of pro-inflammatory cytokines, such as TNF-α- and IL-6, compared with macrophages alone. Therefore, these results suggest that apoptotic neutrophils caused by T. vaginalis may lead to the resolution of vaginal inflammation by anti-inflammatory cytokine production in the human macrophages.

Original languageEnglish
Pages (from-to)410-416
Number of pages7
JournalParasite Immunology
Volume30
Issue number8
DOIs
StatePublished - 2008 Aug 1

Fingerprint

Trichomonas vaginalis
Neutrophils
Anti-Inflammatory Agents
Macrophages
Apoptosis
Cytokines
Trichomonas Infections
Inflammation
Vaginal Discharge
Phagocytosis
Caspase 3
Interleukin-10
Peroxidase
Interleukin-6
Leukocytes
Coloring Agents
Enzyme-Linked Immunosorbent Assay

Keywords

  • Anti-inflammatory cytokine
  • Apoptotic neutrophil
  • Macrophage
  • Phagocytosis
  • Trichomonas vaginalis

Cite this

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abstract = "Neutrophils are the predominant inflammatory cells found in the vaginal discharge of patients with a Trichomonas vaginalis infection. Neutrophils have a shorter life span than other leucocytes. Our previous study indicated that live T. vaginalis alters Mcl-1 expression and caspase-3 activation, thereby inducing apoptosis of human neutrophils. However, it was previously unknown that the apoptotic neutrophils brought about by T. vaginalis can influence vaginal inflammation. Thus, human monocyte-derived macrophages (HMDM) were incubated with T. vaginalis-induced apoptotic neutrophils. Cytokine production and phagocytosis by HMDM were evaluated by ELISA and myeloperoxidase stain, respectively. HMDM showed increased anti-inflammatory cytokine production (IL-10) and decreased levels of pro-inflammatory cytokines, such as TNF-α- and IL-6, compared with macrophages alone. Therefore, these results suggest that apoptotic neutrophils caused by T. vaginalis may lead to the resolution of vaginal inflammation by anti-inflammatory cytokine production in the human macrophages.",
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Trichomonas vaginalis-induced neutrophil apoptosis causes anti-inflammatory cytokine production by human monocyte-derived macrophages. / Ahn, M. H.; Song, H. O.; Ryu, J. S.

In: Parasite Immunology, Vol. 30, No. 8, 01.08.2008, p. 410-416.

Research output: Contribution to journalArticle

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