Time-resolved structural investigation of regenerated silk fibroin nanofibers treated with solvent vapor

Lim Jeong, Kuen Yong Lee, Ju Whan Liu, Won Ho Park

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Nonwoven matrices of silk fibroin (SF) nanofibers were prepared by electrospinning a regenerated SF solution, followed by treatment with solvent vapor including water, methanol, ethanol, and propanol. Structural changes of solvent vapor-treated SF nanofibers were investigated in a time-resolved manner using IR spectroscopy. Conformational transitions of SF nanofibers from random coil to β-sheet forms were dependent on the type of solvent vapor used, and their transition rates were strongly influenced by treatment temperatures. Consistent with previous findings, methanol vapor treatment provided a fast and effective means by which to alter the secondary structure of SF nanofibers. However, treatment with water vapor, as compared to treatment with alcohol vapor, was also useful for inducing structural changes in SF nanofibers. As demonstrated in the present study, our approach of controlling secondary structure formation of proteins by solvent vapor treatment and monitoring real-time conformational changes may be useful for the design and tailoring of materials for biomedical applications.

Original languageEnglish
Pages (from-to)140-144
Number of pages5
JournalInternational Journal of Biological Macromolecules
Volume38
Issue number2
DOIs
StatePublished - 2006 Mar 30

Fingerprint

Fibroins
Nanofibers
Silk
Vapors
Steam
Methanol
Secondary Protein Structure
1-Propanol
Water Purification
Electrospinning
Water vapor
Infrared spectroscopy
Spectrum Analysis
Ethanol
Alcohols
Temperature
Monitoring

Keywords

  • Electrospun nanofiber
  • Silk fibroin
  • Solvent vapor
  • Structural change

Cite this

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abstract = "Nonwoven matrices of silk fibroin (SF) nanofibers were prepared by electrospinning a regenerated SF solution, followed by treatment with solvent vapor including water, methanol, ethanol, and propanol. Structural changes of solvent vapor-treated SF nanofibers were investigated in a time-resolved manner using IR spectroscopy. Conformational transitions of SF nanofibers from random coil to β-sheet forms were dependent on the type of solvent vapor used, and their transition rates were strongly influenced by treatment temperatures. Consistent with previous findings, methanol vapor treatment provided a fast and effective means by which to alter the secondary structure of SF nanofibers. However, treatment with water vapor, as compared to treatment with alcohol vapor, was also useful for inducing structural changes in SF nanofibers. As demonstrated in the present study, our approach of controlling secondary structure formation of proteins by solvent vapor treatment and monitoring real-time conformational changes may be useful for the design and tailoring of materials for biomedical applications.",
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Time-resolved structural investigation of regenerated silk fibroin nanofibers treated with solvent vapor. / Jeong, Lim; Lee, Kuen Yong; Liu, Ju Whan; Park, Won Ho.

In: International Journal of Biological Macromolecules, Vol. 38, No. 2, 30.03.2006, p. 140-144.

Research output: Contribution to journalArticle

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AU - Jeong, Lim

AU - Lee, Kuen Yong

AU - Liu, Ju Whan

AU - Park, Won Ho

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AB - Nonwoven matrices of silk fibroin (SF) nanofibers were prepared by electrospinning a regenerated SF solution, followed by treatment with solvent vapor including water, methanol, ethanol, and propanol. Structural changes of solvent vapor-treated SF nanofibers were investigated in a time-resolved manner using IR spectroscopy. Conformational transitions of SF nanofibers from random coil to β-sheet forms were dependent on the type of solvent vapor used, and their transition rates were strongly influenced by treatment temperatures. Consistent with previous findings, methanol vapor treatment provided a fast and effective means by which to alter the secondary structure of SF nanofibers. However, treatment with water vapor, as compared to treatment with alcohol vapor, was also useful for inducing structural changes in SF nanofibers. As demonstrated in the present study, our approach of controlling secondary structure formation of proteins by solvent vapor treatment and monitoring real-time conformational changes may be useful for the design and tailoring of materials for biomedical applications.

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