Three-Dimensional Bioprinting of Hepatic Structures with Directly Converted Hepatocyte-Like Cells

Kyojin Kang, Yohan Kim, Hyeryeon Jeon, Seung Bum Lee, Ji Sook Kim, Su A. Park, Wan Doo Kim, Heung Mo Yang, Sung Joo Kim, Jaemin Jeong, Dongho Choi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Three-dimensional (3D) bioprinting technology is a promising new technology in the field of bioartificial organ generation with regard to overcoming the limitations of organ supply. The cell source for bioprinting is very important. Here, we generated 3D hepatic scaffold with mouse-induced hepatocyte-like cells (miHeps), and investigated whether their function was improved after transplantation in vivo. To generate miHeps, mouse embryonic fibroblasts (MEFs) were transformed with pMX retroviruses individually expressing hepatic transcription factors Hnf4a and Foxa3. After 8-10 days, MEFs formed rapidly growing hepatocyte-like colonies. For 3D bioprinting, miHeps were mixed with a 3% alginate hydrogel and extruded by nozzle pressure. After 7 days, they were transplanted into the omentum of Jo2-treated NOD Scid gamma (NSG) mice as a liver damage model. Real-time polymerase chain reaction and immunofluorescence analyses were conducted to evaluate hepatic function. The 3D bioprinted hepatic scaffold (25 × 25 mm) expressed Albumin, and ASGR1 and HNF4a expression gradually increased for 28 days in vitro. When transplanted in vivo, the cells in the hepatic scaffold grew more and exhibited higher Albumin expression than in vitro scaffold. Therefore, combining 3D bioprinting with direct conversion technology appears to be an effective option for liver therapy.

Original languageEnglish
Pages (from-to)576-583
Number of pages8
JournalTissue Engineering - Part A
Volume24
Issue number7-8
DOIs
StatePublished - 2018 Apr 1

Fingerprint

Bioprinting
Scaffolds
Hepatocytes
Liver
Fibroblasts
Albumins
Technology
Transcription factors
Bioartificial Organs
Hydrogel
Polymerase chain reaction
Alginate
Hydrogels
Nozzles
Omentum
Transcription Factors
Retroviridae
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Transplantation

Keywords

  • 3D bioprinting
  • Cell transplantation
  • Direct conversion
  • MiHep cells

Cite this

Kang, Kyojin ; Kim, Yohan ; Jeon, Hyeryeon ; Lee, Seung Bum ; Kim, Ji Sook ; Park, Su A. ; Kim, Wan Doo ; Yang, Heung Mo ; Kim, Sung Joo ; Jeong, Jaemin ; Choi, Dongho. / Three-Dimensional Bioprinting of Hepatic Structures with Directly Converted Hepatocyte-Like Cells. In: Tissue Engineering - Part A. 2018 ; Vol. 24, No. 7-8. pp. 576-583.
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Kang, K, Kim, Y, Jeon, H, Lee, SB, Kim, JS, Park, SA, Kim, WD, Yang, HM, Kim, SJ, Jeong, J & Choi, D 2018, 'Three-Dimensional Bioprinting of Hepatic Structures with Directly Converted Hepatocyte-Like Cells', Tissue Engineering - Part A, vol. 24, no. 7-8, pp. 576-583. https://doi.org/10.1089/ten.tea.2017.0161

Three-Dimensional Bioprinting of Hepatic Structures with Directly Converted Hepatocyte-Like Cells. / Kang, Kyojin; Kim, Yohan; Jeon, Hyeryeon; Lee, Seung Bum; Kim, Ji Sook; Park, Su A.; Kim, Wan Doo; Yang, Heung Mo; Kim, Sung Joo; Jeong, Jaemin; Choi, Dongho.

In: Tissue Engineering - Part A, Vol. 24, No. 7-8, 01.04.2018, p. 576-583.

Research output: Contribution to journalArticle

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AU - Kang, Kyojin

AU - Kim, Yohan

AU - Jeon, Hyeryeon

AU - Lee, Seung Bum

AU - Kim, Ji Sook

AU - Park, Su A.

AU - Kim, Wan Doo

AU - Yang, Heung Mo

AU - Kim, Sung Joo

AU - Jeong, Jaemin

AU - Choi, Dongho

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