The involvement of nitric oxide on the adenosine A2 receptor-induced cardiovascular inhibitory responses in the posterior hypothalamus of rats

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Abstract

This study was performed to investigate the putative relationship between nitric oxide (NO) and adenosine A2 receptors on central cardiovascular regulation in the posterior hypothalamus of rats. Posterior hypothalamic injection of drugs was performed in anesthetized, artificially ventilated male Sprague-Dawley rats. Injection of adenosine A2 receptor agonist 5′-(N-cyclopropyl)-carboxamidoadenosine (CPCA; 1, 2 and 5 nmol) produced a dose-dependent decrease of blood pressure and heart rate. Pretreatment with adenosine A2 receptor antagonist 3,7-dimethyl-1-propargylxanthine (10 nmol) blocked the depressor and bradycardiac effects of CPCA (5 nmol). Pretreatment with soluble guanylate cyclase inhibitor LY-83,583 (5 nmol) attenuated the depressor and bradycardiac effects of CPCA (5 nmol). In addition, pretreatment with NO synthase inhibitor NG-nitro-L-arginine methyl ester (40 nmol) attenuated the depressor and bradycardiac responses of CPCA (5 nmol). These results suggest that adenosine A2 receptor in the posterior hypothalamus plays an inhibitory role in central cardiovascular regulation and that NO participates in the inhibitory response induced by adenosine A2 receptor stimulation in the posterior hypothalamus.

Original languageEnglish
Pages (from-to)41-45
Number of pages5
JournalNeuroscience Letters
Volume326
Issue number1
DOIs
StatePublished - 2002 Jun 21

Keywords

  • Adenosine A receptor
  • Blood pressure
  • Guanylate cyclase
  • Heart rate
  • Nitric oxide
  • Posterior hypothalamus

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