The effect of mineral trioxide aggregate on odontogenic differentiation in dental pulp stem cells

Min Seock Seo, Kyung-Gyun Hwang, Jaeseon Lee, Hyongbum Kim, Seung Ho Baek

Research output: Contribution to journalArticle

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Abstract

Introduction: This study aimed to identify the early genetic changes related to odontogenic differentiation when mineral trioxide aggregate (MTA) is applied to dental pulp stem cells (DPSCs). Methods: Odontogenic-differentiated cells (induced DPSCs) were obtained by culturing DPSCs in odontoinduction medium for 14 days. Thereafter, MTA in Teflon tubes was applied to the induced DPSCs and uninduced cells. Cells exposed to empty tubes were used as negative controls. The total RNA was extracted from the MTA treated and MTA untreated cells 1 and 3 days after tube application and assessed by microarray analysis. The key results were confirmed selectively by reverse-transcription polymerase chain reaction. We also performed a gene set enrichment analysis. Results: In microarray analysis, although the expression levels of 460 genes were changed more than 2-fold in MTA-treated, uninduced DPSCs after 1 day, only 39 genes were altered in MTA-treated, induced DPSCs. In the odontoinduction medium-induced, MTA-treated DPCs, the value of correlation was 0.993 on 1 day and 0.986 on 3 day compared with 0.970 on 1 day and 0.975 on 3 day in the uninduced, MTA-treated DPSCs. Gene set enrichment analysis revealed that MTA significantly up-regulated gene sets involved in cell migration, the response to transforming growth factor β1, and the inflammation pathway in the uninduced DPSCs, whereas in the induced DPSCs it only up-regulated genes involved in cell migration after 1 day. Conclusions: This result shows that MTA stimulates the odontogenic differentiation of DPSCs, and the effects of MTA are drastically increased in uninduced pulp cells compared with odontogenic-differentiated cells.

Original languageEnglish
Pages (from-to)242-248
Number of pages7
JournalJournal of Endodontics
Volume39
Issue number2
DOIs
StatePublished - 2013 Feb 1

Fingerprint

Dental Pulp
Stem Cells
Genes
Microarray Analysis
Cell Movement
mineral trioxide aggregate
Polytetrafluoroethylene
Transforming Growth Factors
Reverse Transcription
RNA

Keywords

  • Gene set enrichment analysis
  • human dental pulp cell
  • microarray
  • mineral trioxide aggregate
  • odontoinduction medium

Cite this

Seo, Min Seock ; Hwang, Kyung-Gyun ; Lee, Jaeseon ; Kim, Hyongbum ; Baek, Seung Ho. / The effect of mineral trioxide aggregate on odontogenic differentiation in dental pulp stem cells. In: Journal of Endodontics. 2013 ; Vol. 39, No. 2. pp. 242-248.
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The effect of mineral trioxide aggregate on odontogenic differentiation in dental pulp stem cells. / Seo, Min Seock; Hwang, Kyung-Gyun; Lee, Jaeseon; Kim, Hyongbum; Baek, Seung Ho.

In: Journal of Endodontics, Vol. 39, No. 2, 01.02.2013, p. 242-248.

Research output: Contribution to journalArticle

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