Many experiments investigated the neuro modulatory effects of ultrasonic stimulation, yet there are few researches that investigate which parameters are needed to change activity of excitable cells. In this study, changes in neural network activities were recorded during ultrasonic stimulation with various parameters using a multi-electrode array (MEA). This study concludes, ultrasonic stimulation with intensity of 16.14 mW/cm2 induces change to the neuronal network activity. However, the timing of neuronal activity and stimulus was not synchronized. The mechanical stimuli may not have a direct effect on neuronal activities but somehow help neuronal cells to be in a more excitable state, although further study is necessary.