Survey of extended-spectrum β-lactamases in clinical isolates of Escherichia coli and Klebsiella pneumoniae: Prevalence of TEM-52 in Korea

Hyunjoo Pai, Sen Lyu, Ji Hyang Lee, Jungmin Kim, Youngmi Kwon, Jong Won Kim, Kang Won Choe

Research output: Contribution to journalArticle

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Abstract

Two hundred ninety isolates of Escherichia coli were investigated for the production of extended-spectrum β-lactamases (ESBLs). Fourteen (4.8%) of the 290 strains were found to produce ESBLs. Each of the 14 strains produced one or two ESBLs, as follows: 10 strains produced TEM-52, 1 strain produced SHV-2a, 1 strain produced SHV-12, 1 strain produced a CMY-1-like enzyme, and 1 strain expressed SHV-2a and a CMY-1-like enzyme. Another two strains for which the MICs of ceftazidime and cefoxitin were high, were probable AmpC enzyme hyperproducers. Because of the high prevalence of TEM-52 in E. coli isolates, we further investigated the TEM-type ESBLs produced by Klebsiella pneumoniae in order to observe the distribution of TEM-52 enzymes among Enterobacteriaceae in Korea. All TEM enzymes produced by 12 strains of K. pneumoniae were identified as TEM-52. To evaluate the genetic relatedness among the organisms, ribotyping of TEM-52-producing E. coli and K. pneumoniae was performed. The ribotyping profiles of the organisms showed similar but clearly different patterns. In conclusion, TEM-52 is the most prevalent TEM- type ESBL in Korea.

Original languageEnglish
Pages (from-to)1758-1763
Number of pages6
JournalJournal of Clinical Microbiology
Volume37
Issue number6
StatePublished - 1999 May 29

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Klebsiella pneumoniae
Korea
Escherichia coli
Ribotyping
Enzymes
Cefoxitin
Ceftazidime
Enterobacteriaceae
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Pai, Hyunjoo ; Lyu, Sen ; Lee, Ji Hyang ; Kim, Jungmin ; Kwon, Youngmi ; Kim, Jong Won ; Choe, Kang Won. / Survey of extended-spectrum β-lactamases in clinical isolates of Escherichia coli and Klebsiella pneumoniae : Prevalence of TEM-52 in Korea. In: Journal of Clinical Microbiology. 1999 ; Vol. 37, No. 6. pp. 1758-1763.
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abstract = "Two hundred ninety isolates of Escherichia coli were investigated for the production of extended-spectrum β-lactamases (ESBLs). Fourteen (4.8{\%}) of the 290 strains were found to produce ESBLs. Each of the 14 strains produced one or two ESBLs, as follows: 10 strains produced TEM-52, 1 strain produced SHV-2a, 1 strain produced SHV-12, 1 strain produced a CMY-1-like enzyme, and 1 strain expressed SHV-2a and a CMY-1-like enzyme. Another two strains for which the MICs of ceftazidime and cefoxitin were high, were probable AmpC enzyme hyperproducers. Because of the high prevalence of TEM-52 in E. coli isolates, we further investigated the TEM-type ESBLs produced by Klebsiella pneumoniae in order to observe the distribution of TEM-52 enzymes among Enterobacteriaceae in Korea. All TEM enzymes produced by 12 strains of K. pneumoniae were identified as TEM-52. To evaluate the genetic relatedness among the organisms, ribotyping of TEM-52-producing E. coli and K. pneumoniae was performed. The ribotyping profiles of the organisms showed similar but clearly different patterns. In conclusion, TEM-52 is the most prevalent TEM- type ESBL in Korea.",
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Survey of extended-spectrum β-lactamases in clinical isolates of Escherichia coli and Klebsiella pneumoniae : Prevalence of TEM-52 in Korea. / Pai, Hyunjoo; Lyu, Sen; Lee, Ji Hyang; Kim, Jungmin; Kwon, Youngmi; Kim, Jong Won; Choe, Kang Won.

In: Journal of Clinical Microbiology, Vol. 37, No. 6, 29.05.1999, p. 1758-1763.

Research output: Contribution to journalArticle

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