Suppressed production of pro-inflammatory cytokines by LPS-activated macrophages after treatment with Toxoplasma gondii lysate

Eun Jung Lee, Yoo Mi Heo, Jong Hak Choi, Hyun Ouk Song, Jae Sook Ryu, Myoung Hee Ahn

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

During Toxoplasma gondii infection, macrophages, dendritic cells, and neutrophils are important sources of pro-inflammatory cytokines from the host. To counteract the pro-inflammatory activities, T. gondii is known to have several mechanisms inducing down-regulation of the host immunity. In the present study, we analyzed the production of proand anti-inflammatory cytokines from a human myelomonocytic cell line, THP-1 cells, in response to treatment with T. gondii lysate or lipopolysaccharide (LPS). Treatment of THP-1 cells with LPS induced production of IL-12, TNF-α, IL-8, and IL-10. Co-treatment of THP-1 cells with T. gondii lysate inhibited the LPS-induced IL-12, IL-8 and TNF-α expression, but increased the level of IL-10 synergistically. IL-12 and IL-10 production was down-regulated by anti-human toll-like receptor (TLR)-2 and TLR4 antibodies. T. gondii lysate triggered nuclear factor (NF)-κB-dependent IL-8 expression in HEK293 cells transfected with TLR2. It is suggested that immunosuppression induced by T. gondii lysate treatment might occur via TLR2-mediated NF-κB activation.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalKorean Journal of Parasitology
Volume46
Issue number3
DOIs
StatePublished - 2008 Dec 1

Keywords

  • Cytokine
  • LPS
  • Macrophage
  • NF-κB
  • Toll-like receptor
  • Toxoplasma gondii

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