Sublithographic vertical gold nanogap for label-free electrical detection of protein-ligand binding

Dong Yoon Jang, Young Pil Kim, Hak Sung Kim, Sang Hee Ko Park, Sung Yool Choi, Yang Kyu Choi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Label-free electrical detection of protein-ligand binding using a vertical gold nanogap is presented. A sublithographic nanogap was created using a sacrificial ultrathin film deposited by atomic layer deposition (ALD) in a process similar to the formation of a cantilever in microelectromechanical system processing. Due to the atomic precision of the sacrificial Al 2O3 thickness by ALD, a 7 nm nanogap was successfully fabricated. After binding streptavidin to biotin on the gold surface, an electrical current was measured for various voltages. A dramatic current increase was observed in the case of biotin-streptavidin binding in comparison with the other two cases: a control group filled with air and a biotin-only binding group. There was a minimal current change in the cases of the biotin-PBST group, the biotin-BSA group, and the biotin-saturated streptavidin group, as compared with the biotin-streptavidin group. At a 0.1 μg/ml concentration of streptavidin (1.5 nM), the current difference before and after the protein binding was amplified by approximately 3000-fold with 17 nm nanogap. Also, the detection sensitivity of the vertical nanogap as the gap size varied was investigated. As the size of biotin-streptavidin binding is the most comparable to 12 nm nanogap, the highest sensitivity was shown in the 12 nm gap device. 7 nm nanogap can be used to detect smaller size of biomolecule than that of biotin streptavidin. This arrayable, two-terminal microdevice could be tested for use on a wide range of other biomolecules.

Original languageEnglish
Pages (from-to)443-447
Number of pages5
JournalJournal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures
Volume25
Issue number2
DOIs
StatePublished - 2007 Apr 8

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biotin
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Gold
Ligands
gold
proteins
Proteins
ligands
Atomic layer deposition
Biomolecules
atomic layer epitaxy
Biotin
Ultrathin films
microelectromechanical systems
MEMS
sensitivity
air

Cite this

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abstract = "Label-free electrical detection of protein-ligand binding using a vertical gold nanogap is presented. A sublithographic nanogap was created using a sacrificial ultrathin film deposited by atomic layer deposition (ALD) in a process similar to the formation of a cantilever in microelectromechanical system processing. Due to the atomic precision of the sacrificial Al 2O3 thickness by ALD, a 7 nm nanogap was successfully fabricated. After binding streptavidin to biotin on the gold surface, an electrical current was measured for various voltages. A dramatic current increase was observed in the case of biotin-streptavidin binding in comparison with the other two cases: a control group filled with air and a biotin-only binding group. There was a minimal current change in the cases of the biotin-PBST group, the biotin-BSA group, and the biotin-saturated streptavidin group, as compared with the biotin-streptavidin group. At a 0.1 μg/ml concentration of streptavidin (1.5 nM), the current difference before and after the protein binding was amplified by approximately 3000-fold with 17 nm nanogap. Also, the detection sensitivity of the vertical nanogap as the gap size varied was investigated. As the size of biotin-streptavidin binding is the most comparable to 12 nm nanogap, the highest sensitivity was shown in the 12 nm gap device. 7 nm nanogap can be used to detect smaller size of biomolecule than that of biotin streptavidin. This arrayable, two-terminal microdevice could be tested for use on a wide range of other biomolecules.",
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Sublithographic vertical gold nanogap for label-free electrical detection of protein-ligand binding. / Jang, Dong Yoon; Kim, Young Pil; Kim, Hak Sung; Park, Sang Hee Ko; Choi, Sung Yool; Choi, Yang Kyu.

In: Journal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures, Vol. 25, No. 2, 08.04.2007, p. 443-447.

Research output: Contribution to journalArticle

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T1 - Sublithographic vertical gold nanogap for label-free electrical detection of protein-ligand binding

AU - Jang, Dong Yoon

AU - Kim, Young Pil

AU - Kim, Hak Sung

AU - Park, Sang Hee Ko

AU - Choi, Sung Yool

AU - Choi, Yang Kyu

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