Simultaneous imaging of confocal fluorescence and Raman spectrum

Myoung Ki Ahn, Taehoon Kim, Hongki Yoo, In Cheon Song, Dae Gab Gweon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The advantage of confocal fluorescence microscopy is the ability to acquire high resolution images of fluorescent specimens non-invasively. On the other hand, the advantage of Raman microscopy is the ability to provide the chemical characteristics of specimens from spectroscopy. To obtain simultaneously high resolution images and chemical characteristics of specimens, fluorescence signals from stained cell and Raman spectrum from cell itself should be separated. By separating two kinds of signals, confocal fluorescence image and Raman spectrum are acquired simultaneously at the same position. In this paper, we demonstrate a confocal fluorescence microscopy combined with the Raman microscopy. And we propose a method that eliminates Raman spectrum of fluorophore itself from Raman spectrum of the stained cell and that obtains simultaneously confocal fluorescence image from stained cell and Raman spectrum of cell itself without replacing a cell.

Original languageEnglish
Title of host publicationEuropean Conference on Biomedical Optics, ECBO 2007
PublisherOptical Society of America
ISBN (Print)9780819467713
StatePublished - 2007 Jan 1
EventEuropean Conference on Biomedical Optics, ECBO 2007 - Munich, Germany
Duration: 2007 Jun 172007 Jun 17

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Other

OtherEuropean Conference on Biomedical Optics, ECBO 2007
CountryGermany
CityMunich
Period07/06/1707/06/17

Fingerprint

Raman scattering
Fluorescence
Raman spectra
Imaging techniques
fluorescence
microscopy
cells
Confocal microscopy
Fluorescence microscopy
Image resolution
Microscopic examination
Fluorophores
high resolution
Cells
Spectroscopy
spectroscopy

Keywords

  • Combined confocal fluorescence and raman microscopy
  • Confocal fluorescence image
  • Confocal fluorescence microscopy
  • Raman image
  • Raman microscopy
  • Raman spectrum
  • Simultaneous imaging

Cite this

Ahn, M. K., Kim, T., Yoo, H., Song, I. C., & Gweon, D. G. (2007). Simultaneous imaging of confocal fluorescence and Raman spectrum. In European Conference on Biomedical Optics, ECBO 2007 (Optics InfoBase Conference Papers). Optical Society of America.
Ahn, Myoung Ki ; Kim, Taehoon ; Yoo, Hongki ; Song, In Cheon ; Gweon, Dae Gab. / Simultaneous imaging of confocal fluorescence and Raman spectrum. European Conference on Biomedical Optics, ECBO 2007. Optical Society of America, 2007. (Optics InfoBase Conference Papers).
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Ahn, MK, Kim, T, Yoo, H, Song, IC & Gweon, DG 2007, Simultaneous imaging of confocal fluorescence and Raman spectrum. in European Conference on Biomedical Optics, ECBO 2007. Optics InfoBase Conference Papers, Optical Society of America, European Conference on Biomedical Optics, ECBO 2007, Munich, Germany, 07/06/17.

Simultaneous imaging of confocal fluorescence and Raman spectrum. / Ahn, Myoung Ki; Kim, Taehoon; Yoo, Hongki; Song, In Cheon; Gweon, Dae Gab.

European Conference on Biomedical Optics, ECBO 2007. Optical Society of America, 2007. (Optics InfoBase Conference Papers).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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N2 - The advantage of confocal fluorescence microscopy is the ability to acquire high resolution images of fluorescent specimens non-invasively. On the other hand, the advantage of Raman microscopy is the ability to provide the chemical characteristics of specimens from spectroscopy. To obtain simultaneously high resolution images and chemical characteristics of specimens, fluorescence signals from stained cell and Raman spectrum from cell itself should be separated. By separating two kinds of signals, confocal fluorescence image and Raman spectrum are acquired simultaneously at the same position. In this paper, we demonstrate a confocal fluorescence microscopy combined with the Raman microscopy. And we propose a method that eliminates Raman spectrum of fluorophore itself from Raman spectrum of the stained cell and that obtains simultaneously confocal fluorescence image from stained cell and Raman spectrum of cell itself without replacing a cell.

AB - The advantage of confocal fluorescence microscopy is the ability to acquire high resolution images of fluorescent specimens non-invasively. On the other hand, the advantage of Raman microscopy is the ability to provide the chemical characteristics of specimens from spectroscopy. To obtain simultaneously high resolution images and chemical characteristics of specimens, fluorescence signals from stained cell and Raman spectrum from cell itself should be separated. By separating two kinds of signals, confocal fluorescence image and Raman spectrum are acquired simultaneously at the same position. In this paper, we demonstrate a confocal fluorescence microscopy combined with the Raman microscopy. And we propose a method that eliminates Raman spectrum of fluorophore itself from Raman spectrum of the stained cell and that obtains simultaneously confocal fluorescence image from stained cell and Raman spectrum of cell itself without replacing a cell.

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Ahn MK, Kim T, Yoo H, Song IC, Gweon DG. Simultaneous imaging of confocal fluorescence and Raman spectrum. In European Conference on Biomedical Optics, ECBO 2007. Optical Society of America. 2007. (Optics InfoBase Conference Papers).