SERS-based genetic assay for amplification-free detection of prostate cancer specific PCA3 mimic DNA

Jimin Yu, Jinhyeok Jeon, Namhyun Choi, Jin Oh Lee, Young Pil Kim, Jaebum Choo

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

We report the development of amplification-free surface-enhanced Raman scattering (SERS)-based DNA assays for the rapid and highly sensitive detection of prostate cancer antigen 3 (PCA3) mimic DNA. This technique does not require any DNA amplification process using thermo-cycles in conventional polymerase chain reaction (PCR) due to its highly sensitive detection capability. Herein, the PCA3 mimic DNA, composed of 45 nucleotide sequences, was sandwiched between two probe DNA-immobilized particles (ASO735-conjugated detection HGNs and ASO683-immobilized capture magnetic beads) by hybridization reactions. Its quantitative analysis was performed by monitoring the characteristic Raman peak intensity of sandwich DNA complexes. The limit of detection (LOD) is estimated to be 2.7 fM, which is about four orders of magnitude more sensitive than that of conventional PCR. This SERS-based DNA assay technique is expected to be a potentially useful tool for early disease diagnosis.

Original languageEnglish
Pages (from-to)302-309
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume251
DOIs
StatePublished - 2017 Jan 1

Keywords

  • Amplification-free DNA detection
  • Hollow gold nanospheres
  • Polymerase chain reaction (PCR)
  • Prostate cancer antigen 3 (PCA3)
  • Surface-enhanced Raman scattering

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