Sensitivity evaluation of rhodamine B hydrazide towards nitric oxide and its application for macrophage cells imaging

Chi Ming Wu, Yen Hao Chen, Kasala Dayananda, Tsun Wei Shiue, Chen Hsiung Hung, Wen Feng Liaw, Po Yu Chen, Yun Ming Wang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A colorless and non-fluorescent rhodamine derivative, rhodamine B hydrazide (RH), is applied to detect nitric oxide and form fluorescent rhodamine B (RB). The reaction mechanism of RH with NO is proposed in this study. The probe shows good stability over a broad pH range (pH > 4). Furthermore, fluorescence intensity of RH displays an excellent linearity to the NO concentration and the detection limit is as low as 20. nM. A 1000-fold fluorescence turn-on from a dark background was observed. Moreover, the selectivity study indicated that the fluorescence intensity increasing in the presence of NO was significantly higher than those of other reactive oxygen/nitrogen species. In exogenously generated NO detection study, clear intracellular red fluorescence was observed in the presence of S-nitroso- N-acetyl-D,L-penicillamine (SNAP, a kind of NO releasing agent). In endogenously generated NO detection study, increasing incubation time of RH with lipopolysaccharied (LPS) pre-treated cells could obtain a highly fluorescent cell image. These cell imaging results demonstrated that RH can efficiently penetrate into Raw 264.7 cells and be used for detection of exogenously and endogenously generated nitric oxide.

Original languageEnglish
Pages (from-to)141-148
Number of pages8
JournalAnalytica Chimica Acta
Volume708
Issue number1-2
DOIs
StatePublished - 2011 Dec 5

Fingerprint

Macrophages
nitric oxide
Nitric Oxide
fluorescence
Imaging techniques
Fluorescence
rhodamine B
Reactive Nitrogen Species
linearity
Penicillamine
Rhodamines
incubation
probe
Limit of Detection
fold
Reactive Oxygen Species
Nitrogen
oxygen
Cells
rhodamine B hydrazide

Keywords

  • Cell imaging
  • Fluorescent probe
  • Nitric oxide
  • Rhodamine

Cite this

Wu, Chi Ming ; Chen, Yen Hao ; Dayananda, Kasala ; Shiue, Tsun Wei ; Hung, Chen Hsiung ; Liaw, Wen Feng ; Chen, Po Yu ; Wang, Yun Ming. / Sensitivity evaluation of rhodamine B hydrazide towards nitric oxide and its application for macrophage cells imaging. In: Analytica Chimica Acta. 2011 ; Vol. 708, No. 1-2. pp. 141-148.
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Sensitivity evaluation of rhodamine B hydrazide towards nitric oxide and its application for macrophage cells imaging. / Wu, Chi Ming; Chen, Yen Hao; Dayananda, Kasala; Shiue, Tsun Wei; Hung, Chen Hsiung; Liaw, Wen Feng; Chen, Po Yu; Wang, Yun Ming.

In: Analytica Chimica Acta, Vol. 708, No. 1-2, 05.12.2011, p. 141-148.

Research output: Contribution to journalArticle

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