Sensitive and multiplexed analysis of aflatoxins using time-of-flight secondary ion mass spectrometry

Jang Hyuk Ahn, Young Su Jeong, Tae Geol Lee, Young-Pil Kim, Hak Sung Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A rapid and reliable analysis of toxins is prerequisite for food control and human healthcare. Here we demonstrate a simple and multiplexed assay of aflatoxins using time-of-flight secondary ion mass spectrometry (TOF-SIMS). By simply adsorbing either a single analyte or mixed ones onto a gold substrate, the corresponding secondary molecular ions([M+H]+) were clearly observed in a single mass spectrum. As a result of concentration-dependent peak intensity, quantitative and multiplexed analysis of different aflatoxin analogs from corns was accomplished with immunoaffinity column and TOF-SIMS analysis, which showed a good correlation with HPLC data. The detection sensitivity was estimated to be as low as 10 ng mL-1. This approach presented here will find a wide application to detection of low-levels of toxins in a rapid and multiplexed way.

Original languageEnglish
Pages (from-to)34-40
Number of pages7
JournalBiochip Journal
Volume6
Issue number1
DOIs
StatePublished - 2012 Jan 1

Fingerprint

Secondary Ion Mass Spectrometry
Aflatoxins
Secondary ion mass spectrometry
Callosities
Gold
Assays
High Pressure Liquid Chromatography
Ions
Delivery of Health Care
Food
Substrates

Keywords

  • Aflatoxin
  • Multiplexed analysis
  • Quantification
  • TOF-SIMS

Cite this

Ahn, Jang Hyuk ; Jeong, Young Su ; Lee, Tae Geol ; Kim, Young-Pil ; Kim, Hak Sung. / Sensitive and multiplexed analysis of aflatoxins using time-of-flight secondary ion mass spectrometry. In: Biochip Journal. 2012 ; Vol. 6, No. 1. pp. 34-40.
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Sensitive and multiplexed analysis of aflatoxins using time-of-flight secondary ion mass spectrometry. / Ahn, Jang Hyuk; Jeong, Young Su; Lee, Tae Geol; Kim, Young-Pil; Kim, Hak Sung.

In: Biochip Journal, Vol. 6, No. 1, 01.01.2012, p. 34-40.

Research output: Contribution to journalArticle

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