Release Properties and Cellular Uptake in Caco-2 Cells of Size-Controlled Chitosan Nanoparticles

Hyun Jeong Je, Eun Suh Kim, Ji Soo Lee, Hyeon Gyu Lee

Research output: Contribution to journalArticle

7 Scopus citations


The influences of particle size on the physicochemical, release, and cellular uptake properties of chitosan nanoparticles (CSNPs) were investigated. Ionotropic CSNPs of different sizes (200-1000 nm) loaded with two model core materials (resveratrol or coumarin-6) were prepared using tripolyphosphate and carrageenan as cross-linkers. With an increase of particle size, zeta potential (34.6 ± 0.5 to 51.1 ± 0.9) and entrapment efficiency (14.9 ± 1.4 to 40.9 ± 1.9) of the CSNPs were significantly (p < 0.05) increased and release rates were decreased. However, Caco-2 cellular uptake of CSNPs were significantly increased from 3.70 ± 0.03 to 5.24 ± 0.20 with an increase of particle size from 200 to 600 nm, whereas those significantly decreased from 5.24 ± 0.20 to 4.55 ± 0.2 for particles larger than 600 nm in transwell assay. Moreover, much the same uptake patterns were also observed in confocal microscopy and flow cytometry. Investigation of cellular uptake of CSNPs revealed positive correlations between ZP and EE and indicated the effects of complex factors of nanoparticles other than size. These results provide a better understanding of CSNPs absorption and raises the possibility of controlling alternative nanoparticle properties to enhance bioavailability.

Original languageEnglish
Pages (from-to)10899-10906
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Issue number50
StatePublished - 2017 Dec 20


  • cellular permeability
  • cellular uptake
  • controlled release
  • nanoencapsulation
  • size control

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