Regulator of Calcineurin (RCAN-1) Regulates Thermotaxis Behavior in Caenorhabditis elegans

Weixun Li, Harold W. Bell, Joohong Ahnn, Sun Kyung Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Regulator of calcineurin (RCAN) is a calcineurin-interacting protein that inhibits calcineurin phosphatase when overexpressed, often upregulated under neuropathological conditions with impaired learning and memory processes, such as Down syndrome or Alzheimer's disease. Thermotactic behavior in the nematode Caenorhabditis elegans is a form of memory in which calcineurin signaling plays a pivotal role in the thermosensation of AFD neurons. In this study, we found that rcan-1 deletion mutants exhibited cryophilic behavior dependent on tax-6, which was rescued by expressing rcan-1 in AFD neurons. Interaction between RCAN-1 and TAX-6 requires the conserved PxIxIT motif of RCAN-1, without which thermotactic behavior could not be fully rescued. In addition, the loss of crh-1/CREB suppressed the thermotaxis phenotypes of rcan-1 and tax-6 mutants, indicating that crh-1 is crucial in thermotaxis memory in these mutants. Taken together, our results suggest that rcan-1 is an inhibitory regulator of tax-6 and that it acts in the formation of thermosensory behavioral memory in C. Elegans.

Original languageEnglish
Pages (from-to)3457-3468
Number of pages12
JournalJournal of Molecular Biology
Volume427
Issue number22
DOIs
StatePublished - 2015 Mar 9

Fingerprint

Calcineurin
Caenorhabditis elegans
Neurons
Down Syndrome
Alzheimer Disease
Taxis Response
Learning
Phenotype
Proteins

Keywords

  • CREB
  • Caenorhabditis elegans
  • RCAN
  • calcineurin
  • thermotaxis

Cite this

Li, Weixun ; Bell, Harold W. ; Ahnn, Joohong ; Lee, Sun Kyung. / Regulator of Calcineurin (RCAN-1) Regulates Thermotaxis Behavior in Caenorhabditis elegans. In: Journal of Molecular Biology. 2015 ; Vol. 427, No. 22. pp. 3457-3468.
@article{5860b194fa9f40b08572c45b1327ead3,
title = "Regulator of Calcineurin (RCAN-1) Regulates Thermotaxis Behavior in Caenorhabditis elegans",
abstract = "Regulator of calcineurin (RCAN) is a calcineurin-interacting protein that inhibits calcineurin phosphatase when overexpressed, often upregulated under neuropathological conditions with impaired learning and memory processes, such as Down syndrome or Alzheimer's disease. Thermotactic behavior in the nematode Caenorhabditis elegans is a form of memory in which calcineurin signaling plays a pivotal role in the thermosensation of AFD neurons. In this study, we found that rcan-1 deletion mutants exhibited cryophilic behavior dependent on tax-6, which was rescued by expressing rcan-1 in AFD neurons. Interaction between RCAN-1 and TAX-6 requires the conserved PxIxIT motif of RCAN-1, without which thermotactic behavior could not be fully rescued. In addition, the loss of crh-1/CREB suppressed the thermotaxis phenotypes of rcan-1 and tax-6 mutants, indicating that crh-1 is crucial in thermotaxis memory in these mutants. Taken together, our results suggest that rcan-1 is an inhibitory regulator of tax-6 and that it acts in the formation of thermosensory behavioral memory in C. Elegans.",
keywords = "CREB, Caenorhabditis elegans, RCAN, calcineurin, thermotaxis",
author = "Weixun Li and Bell, {Harold W.} and Joohong Ahnn and Lee, {Sun Kyung}",
year = "2015",
month = "3",
day = "9",
doi = "10.1016/j.jmb.2015.07.017",
language = "English",
volume = "427",
pages = "3457--3468",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
number = "22",

}

Regulator of Calcineurin (RCAN-1) Regulates Thermotaxis Behavior in Caenorhabditis elegans. / Li, Weixun; Bell, Harold W.; Ahnn, Joohong; Lee, Sun Kyung.

In: Journal of Molecular Biology, Vol. 427, No. 22, 09.03.2015, p. 3457-3468.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Regulator of Calcineurin (RCAN-1) Regulates Thermotaxis Behavior in Caenorhabditis elegans

AU - Li, Weixun

AU - Bell, Harold W.

AU - Ahnn, Joohong

AU - Lee, Sun Kyung

PY - 2015/3/9

Y1 - 2015/3/9

N2 - Regulator of calcineurin (RCAN) is a calcineurin-interacting protein that inhibits calcineurin phosphatase when overexpressed, often upregulated under neuropathological conditions with impaired learning and memory processes, such as Down syndrome or Alzheimer's disease. Thermotactic behavior in the nematode Caenorhabditis elegans is a form of memory in which calcineurin signaling plays a pivotal role in the thermosensation of AFD neurons. In this study, we found that rcan-1 deletion mutants exhibited cryophilic behavior dependent on tax-6, which was rescued by expressing rcan-1 in AFD neurons. Interaction between RCAN-1 and TAX-6 requires the conserved PxIxIT motif of RCAN-1, without which thermotactic behavior could not be fully rescued. In addition, the loss of crh-1/CREB suppressed the thermotaxis phenotypes of rcan-1 and tax-6 mutants, indicating that crh-1 is crucial in thermotaxis memory in these mutants. Taken together, our results suggest that rcan-1 is an inhibitory regulator of tax-6 and that it acts in the formation of thermosensory behavioral memory in C. Elegans.

AB - Regulator of calcineurin (RCAN) is a calcineurin-interacting protein that inhibits calcineurin phosphatase when overexpressed, often upregulated under neuropathological conditions with impaired learning and memory processes, such as Down syndrome or Alzheimer's disease. Thermotactic behavior in the nematode Caenorhabditis elegans is a form of memory in which calcineurin signaling plays a pivotal role in the thermosensation of AFD neurons. In this study, we found that rcan-1 deletion mutants exhibited cryophilic behavior dependent on tax-6, which was rescued by expressing rcan-1 in AFD neurons. Interaction between RCAN-1 and TAX-6 requires the conserved PxIxIT motif of RCAN-1, without which thermotactic behavior could not be fully rescued. In addition, the loss of crh-1/CREB suppressed the thermotaxis phenotypes of rcan-1 and tax-6 mutants, indicating that crh-1 is crucial in thermotaxis memory in these mutants. Taken together, our results suggest that rcan-1 is an inhibitory regulator of tax-6 and that it acts in the formation of thermosensory behavioral memory in C. Elegans.

KW - CREB

KW - Caenorhabditis elegans

KW - RCAN

KW - calcineurin

KW - thermotaxis

UR - http://www.scopus.com/inward/record.url?scp=84952864311&partnerID=8YFLogxK

U2 - 10.1016/j.jmb.2015.07.017

DO - 10.1016/j.jmb.2015.07.017

M3 - Article

C2 - 26232604

AN - SCOPUS:84952864311

VL - 427

SP - 3457

EP - 3468

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 22

ER -