Regeneration of textile wastewater deteriorated microbial diversity of soil microcosm through bioaugmentation

Swapnil M. Patil, Mangesh V. Suryavanshi, Vishal V. Chandanshive, Mayur B. Kurade, Sanjay Prabhu Govindwar, Byong-Hun Jeon

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Textile dye contamination is a serious concern that reduces soil productivity by destabilizing microbial community structures. Here, we investigated the influence of bioaugmentation on the degradation of a mixture of dyes (MOD) and textile industry effluent (TIE) in soil microcosms using eight different dye-degrading bacteria. The biodegradation potential improved in bioaugmented microcosms, especially in the initial phase. The bioaugmented MOD and TIE microcosms exhibited 98.33% and 94.19% decolorization, and 96.92% and 95% reduction in chemical oxygen demand, respectively, within 30 days. Activities of azoreductase, veratryl alcohol oxidase, lignin peroxidase, and tyrosinase were induced by >three-fold in bioaugmented microcosms. Changes in alpha diversity indicated significant alterations in microbial dynamics due to MOD and TIE feeding. The Rheinheimera, Kocuria, Ruminococcaceae UCG-010, Ralstonia and Pseudomonas assemblages were predominant after exposure to MOD and TIE, indicating their key role in dye degradation. The bacteria used for augmentation, namely, Staphylococcus, Bacillus, Arthrobacter and Pseudomonas dominantly survived in soil microcosms. Xenobiotic pathways including benzoate, aminobenzoate, chloroalkane and chloroalkene degradation contributed in dye's detoxification as per illustration of functional annotation of metagenomes. This study indicates a mutualistic-symbiotic relationship between augmented bacteria and soil microflora with enhanced detoxification of xenobiotics leading to a sustainable approach for restoration of contaminated lands.

Original languageEnglish
Article number122533
JournalChemical Engineering Journal
Volume380
DOIs
StatePublished - 2020 Jan 15

Fingerprint

microcosm
dye
Textiles
Wastewater
Coloring Agents
Dyes
regeneration
Soils
wastewater
textile industry
Textile industry
Effluents
soil
effluent
Bacteria
Detoxification
Xenobiotics
xenobiotics
detoxification
Degradation

Keywords

  • Bioaugmentation
  • Biodegradation
  • Decolorization
  • Metagenomics
  • Microcosm

Cite this

@article{c2bb57a6729b4d088802ebad9a69ad0c,
title = "Regeneration of textile wastewater deteriorated microbial diversity of soil microcosm through bioaugmentation",
abstract = "Textile dye contamination is a serious concern that reduces soil productivity by destabilizing microbial community structures. Here, we investigated the influence of bioaugmentation on the degradation of a mixture of dyes (MOD) and textile industry effluent (TIE) in soil microcosms using eight different dye-degrading bacteria. The biodegradation potential improved in bioaugmented microcosms, especially in the initial phase. The bioaugmented MOD and TIE microcosms exhibited 98.33{\%} and 94.19{\%} decolorization, and 96.92{\%} and 95{\%} reduction in chemical oxygen demand, respectively, within 30 days. Activities of azoreductase, veratryl alcohol oxidase, lignin peroxidase, and tyrosinase were induced by >three-fold in bioaugmented microcosms. Changes in alpha diversity indicated significant alterations in microbial dynamics due to MOD and TIE feeding. The Rheinheimera, Kocuria, Ruminococcaceae UCG-010, Ralstonia and Pseudomonas assemblages were predominant after exposure to MOD and TIE, indicating their key role in dye degradation. The bacteria used for augmentation, namely, Staphylococcus, Bacillus, Arthrobacter and Pseudomonas dominantly survived in soil microcosms. Xenobiotic pathways including benzoate, aminobenzoate, chloroalkane and chloroalkene degradation contributed in dye's detoxification as per illustration of functional annotation of metagenomes. This study indicates a mutualistic-symbiotic relationship between augmented bacteria and soil microflora with enhanced detoxification of xenobiotics leading to a sustainable approach for restoration of contaminated lands.",
keywords = "Bioaugmentation, Biodegradation, Decolorization, Metagenomics, Microcosm",
author = "Patil, {Swapnil M.} and Suryavanshi, {Mangesh V.} and Chandanshive, {Vishal V.} and Kurade, {Mayur B.} and Govindwar, {Sanjay Prabhu} and Byong-Hun Jeon",
year = "2020",
month = "1",
day = "15",
doi = "10.1016/j.cej.2019.122533",
language = "English",
volume = "380",
journal = "Chemical Engineering Journal",
issn = "1385-8947",

}

Regeneration of textile wastewater deteriorated microbial diversity of soil microcosm through bioaugmentation. / Patil, Swapnil M.; Suryavanshi, Mangesh V.; Chandanshive, Vishal V.; Kurade, Mayur B.; Govindwar, Sanjay Prabhu; Jeon, Byong-Hun.

In: Chemical Engineering Journal, Vol. 380, 122533, 15.01.2020.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Regeneration of textile wastewater deteriorated microbial diversity of soil microcosm through bioaugmentation

AU - Patil, Swapnil M.

AU - Suryavanshi, Mangesh V.

AU - Chandanshive, Vishal V.

AU - Kurade, Mayur B.

AU - Govindwar, Sanjay Prabhu

AU - Jeon, Byong-Hun

PY - 2020/1/15

Y1 - 2020/1/15

N2 - Textile dye contamination is a serious concern that reduces soil productivity by destabilizing microbial community structures. Here, we investigated the influence of bioaugmentation on the degradation of a mixture of dyes (MOD) and textile industry effluent (TIE) in soil microcosms using eight different dye-degrading bacteria. The biodegradation potential improved in bioaugmented microcosms, especially in the initial phase. The bioaugmented MOD and TIE microcosms exhibited 98.33% and 94.19% decolorization, and 96.92% and 95% reduction in chemical oxygen demand, respectively, within 30 days. Activities of azoreductase, veratryl alcohol oxidase, lignin peroxidase, and tyrosinase were induced by >three-fold in bioaugmented microcosms. Changes in alpha diversity indicated significant alterations in microbial dynamics due to MOD and TIE feeding. The Rheinheimera, Kocuria, Ruminococcaceae UCG-010, Ralstonia and Pseudomonas assemblages were predominant after exposure to MOD and TIE, indicating their key role in dye degradation. The bacteria used for augmentation, namely, Staphylococcus, Bacillus, Arthrobacter and Pseudomonas dominantly survived in soil microcosms. Xenobiotic pathways including benzoate, aminobenzoate, chloroalkane and chloroalkene degradation contributed in dye's detoxification as per illustration of functional annotation of metagenomes. This study indicates a mutualistic-symbiotic relationship between augmented bacteria and soil microflora with enhanced detoxification of xenobiotics leading to a sustainable approach for restoration of contaminated lands.

AB - Textile dye contamination is a serious concern that reduces soil productivity by destabilizing microbial community structures. Here, we investigated the influence of bioaugmentation on the degradation of a mixture of dyes (MOD) and textile industry effluent (TIE) in soil microcosms using eight different dye-degrading bacteria. The biodegradation potential improved in bioaugmented microcosms, especially in the initial phase. The bioaugmented MOD and TIE microcosms exhibited 98.33% and 94.19% decolorization, and 96.92% and 95% reduction in chemical oxygen demand, respectively, within 30 days. Activities of azoreductase, veratryl alcohol oxidase, lignin peroxidase, and tyrosinase were induced by >three-fold in bioaugmented microcosms. Changes in alpha diversity indicated significant alterations in microbial dynamics due to MOD and TIE feeding. The Rheinheimera, Kocuria, Ruminococcaceae UCG-010, Ralstonia and Pseudomonas assemblages were predominant after exposure to MOD and TIE, indicating their key role in dye degradation. The bacteria used for augmentation, namely, Staphylococcus, Bacillus, Arthrobacter and Pseudomonas dominantly survived in soil microcosms. Xenobiotic pathways including benzoate, aminobenzoate, chloroalkane and chloroalkene degradation contributed in dye's detoxification as per illustration of functional annotation of metagenomes. This study indicates a mutualistic-symbiotic relationship between augmented bacteria and soil microflora with enhanced detoxification of xenobiotics leading to a sustainable approach for restoration of contaminated lands.

KW - Bioaugmentation

KW - Biodegradation

KW - Decolorization

KW - Metagenomics

KW - Microcosm

UR - http://www.scopus.com/inward/record.url?scp=85070947840&partnerID=8YFLogxK

U2 - 10.1016/j.cej.2019.122533

DO - 10.1016/j.cej.2019.122533

M3 - Article

VL - 380

JO - Chemical Engineering Journal

JF - Chemical Engineering Journal

SN - 1385-8947

M1 - 122533

ER -