Abstract
Adipogenesis inhibitory peptide was isolated and identified from black soybean (Rhynchosia volubilis Lour.) hydrolysate. An adipogenesis inhibitor was purified using consecutive methods including: ultrafiltration (MWCO; 3 and 10 kDa), gel filtration chromatography (Superdex Peptide 10/300 GL column), and reverse-phase high-performance liquid chromatography (μBondapak™ C18 column). Also, the adipogenesis inhibition effect of the purified peptide was measured by observation of droplet of 3T3-L1 adipocyte by Oil Red O staining in the highest active fraction in each step. The peptide was shown to inhibit the differentiation of the 3T3-L1 pre-adipocyte, which was confirmed by morphological study. The adipogenesis inhibitory peptide was purified 71.43-fold from black soybean hydrolysate throughout a five-step purification procedure. The adipogenesis inhibitor was identified to be a tripeptide, Ile-Gln-Asn, having an IC50 value of 0.014 mg protein/ml. Furthermore, the synthetic tripeptide (Ile-Gln-Asn) exhibited the similar adipogenesis effects to the purified peptide. Thus, these results showed the potential anti-obesity effect of the purified peptide through control of adiposity.
| Original language | English |
|---|---|
| Pages (from-to) | 2098-2103 |
| Number of pages | 6 |
| Journal | Peptides |
| Volume | 28 |
| Issue number | 11 |
| DOIs | |
| State | Published - 2007 Nov 1 |
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Keywords
- 3T3-L1 cell
- Adipogenesis inhibitor
- Black soybean peptide
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}
Purification and identification of adipogenesis inhibitory peptide from black soybean protein hydrolysate. / Kim, Hyun Jeong; Bae, In Young; Ahn, Chang Won; Lee, Suyong; Lee, Hyeon Gyu.
In: Peptides, Vol. 28, No. 11, 01.11.2007, p. 2098-2103.Research output: Contribution to journal › Article
TY - JOUR
T1 - Purification and identification of adipogenesis inhibitory peptide from black soybean protein hydrolysate
AU - Kim, Hyun Jeong
AU - Bae, In Young
AU - Ahn, Chang Won
AU - Lee, Suyong
AU - Lee, Hyeon Gyu
PY - 2007/11/1
Y1 - 2007/11/1
N2 - Adipogenesis inhibitory peptide was isolated and identified from black soybean (Rhynchosia volubilis Lour.) hydrolysate. An adipogenesis inhibitor was purified using consecutive methods including: ultrafiltration (MWCO; 3 and 10 kDa), gel filtration chromatography (Superdex Peptide 10/300 GL column), and reverse-phase high-performance liquid chromatography (μBondapak™ C18 column). Also, the adipogenesis inhibition effect of the purified peptide was measured by observation of droplet of 3T3-L1 adipocyte by Oil Red O staining in the highest active fraction in each step. The peptide was shown to inhibit the differentiation of the 3T3-L1 pre-adipocyte, which was confirmed by morphological study. The adipogenesis inhibitory peptide was purified 71.43-fold from black soybean hydrolysate throughout a five-step purification procedure. The adipogenesis inhibitor was identified to be a tripeptide, Ile-Gln-Asn, having an IC50 value of 0.014 mg protein/ml. Furthermore, the synthetic tripeptide (Ile-Gln-Asn) exhibited the similar adipogenesis effects to the purified peptide. Thus, these results showed the potential anti-obesity effect of the purified peptide through control of adiposity.
AB - Adipogenesis inhibitory peptide was isolated and identified from black soybean (Rhynchosia volubilis Lour.) hydrolysate. An adipogenesis inhibitor was purified using consecutive methods including: ultrafiltration (MWCO; 3 and 10 kDa), gel filtration chromatography (Superdex Peptide 10/300 GL column), and reverse-phase high-performance liquid chromatography (μBondapak™ C18 column). Also, the adipogenesis inhibition effect of the purified peptide was measured by observation of droplet of 3T3-L1 adipocyte by Oil Red O staining in the highest active fraction in each step. The peptide was shown to inhibit the differentiation of the 3T3-L1 pre-adipocyte, which was confirmed by morphological study. The adipogenesis inhibitory peptide was purified 71.43-fold from black soybean hydrolysate throughout a five-step purification procedure. The adipogenesis inhibitor was identified to be a tripeptide, Ile-Gln-Asn, having an IC50 value of 0.014 mg protein/ml. Furthermore, the synthetic tripeptide (Ile-Gln-Asn) exhibited the similar adipogenesis effects to the purified peptide. Thus, these results showed the potential anti-obesity effect of the purified peptide through control of adiposity.
KW - 3T3-L1 cell
KW - Adipogenesis inhibitor
KW - Black soybean peptide
UR - http://www.scopus.com/inward/record.url?scp=35348991559&partnerID=8YFLogxK
U2 - 10.1016/j.peptides.2007.08.030
DO - 10.1016/j.peptides.2007.08.030
M3 - Article
C2 - 17935831
AN - SCOPUS:35348991559
VL - 28
SP - 2098
EP - 2103
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 11
ER -