Purification and identification of adipogenesis inhibitory peptide from black soybean protein hydrolysate

Hyun Jeong Kim, In Young Bae, Chang Won Ahn, Suyong Lee, Hyeon Gyu Lee

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Adipogenesis inhibitory peptide was isolated and identified from black soybean (Rhynchosia volubilis Lour.) hydrolysate. An adipogenesis inhibitor was purified using consecutive methods including: ultrafiltration (MWCO; 3 and 10 kDa), gel filtration chromatography (Superdex Peptide 10/300 GL column), and reverse-phase high-performance liquid chromatography (μBondapak™ C18 column). Also, the adipogenesis inhibition effect of the purified peptide was measured by observation of droplet of 3T3-L1 adipocyte by Oil Red O staining in the highest active fraction in each step. The peptide was shown to inhibit the differentiation of the 3T3-L1 pre-adipocyte, which was confirmed by morphological study. The adipogenesis inhibitory peptide was purified 71.43-fold from black soybean hydrolysate throughout a five-step purification procedure. The adipogenesis inhibitor was identified to be a tripeptide, Ile-Gln-Asn, having an IC50 value of 0.014 mg protein/ml. Furthermore, the synthetic tripeptide (Ile-Gln-Asn) exhibited the similar adipogenesis effects to the purified peptide. Thus, these results showed the potential anti-obesity effect of the purified peptide through control of adiposity.

Original languageEnglish
Pages (from-to)2098-2103
Number of pages6
JournalPeptides
Volume28
Issue number11
DOIs
StatePublished - 2007 Nov 1

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Protein Hydrolysates
Adipogenesis
Soybean Proteins
Purification
Peptides
Soybeans
Adipocytes
Adiposity
Ultrafiltration
High performance liquid chromatography
Reverse-Phase Chromatography
Chromatography
Inhibitory Concentration 50
Gel Chromatography
Obesity
Gels
High Pressure Liquid Chromatography
Observation
Staining and Labeling

Keywords

  • 3T3-L1 cell
  • Adipogenesis inhibitor
  • Black soybean peptide

Cite this

Kim, Hyun Jeong ; Bae, In Young ; Ahn, Chang Won ; Lee, Suyong ; Lee, Hyeon Gyu. / Purification and identification of adipogenesis inhibitory peptide from black soybean protein hydrolysate. In: Peptides. 2007 ; Vol. 28, No. 11. pp. 2098-2103.
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Purification and identification of adipogenesis inhibitory peptide from black soybean protein hydrolysate. / Kim, Hyun Jeong; Bae, In Young; Ahn, Chang Won; Lee, Suyong; Lee, Hyeon Gyu.

In: Peptides, Vol. 28, No. 11, 01.11.2007, p. 2098-2103.

Research output: Contribution to journalArticle

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