Proteomic profiling of emiliania huxleyi using a three-dimensional separation method combined with tandem mass spectrometry

Goyeun Yun, Jong Moon Park, Van An Duong, Jeong Hun Mok, Jongho Jeon, Onyou Nam, Joonwon Lee, Eonseon Jin, Hookeun Lee

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Emiliania huxleyi is one of the most abundant marine planktons, and it has a crucial feature in the carbon cycle. However, proteomic analyses of Emiliania huxleyi have not been done extensively. In this study, a three-dimensional liquid chromatography (3D-LC) system consisting of strong cation exchange, high- and low-pH reversed-phase liquid chromatography was established for in-depth proteomic profiling of Emiliania huxleyi. From tryptic proteome digest, 70 fractions were generated and analyzed using liquid chromatography-tandem mass spectrometry. In total, more than 84,000 unique peptides and 10,000 proteins groups were identified with a false discovery rate of ≤0.01. The physicochemical properties of the identified peptides were evaluated. Using ClueGO, approximately 700 gene ontology terms and 15 pathways were defined from the identified protein groups with p-value ≤0.05, covering a wide range of biological processes, cellular components, and molecular functions. Many biological processes associated with CO2fixation, photosynthesis, biosynthesis, and metabolic process were identified. Various molecular functions relating to protein binding and enzyme activities were also found. The 3D-LC strategy is a powerful approach for comparative proteomic studies on Emiliania huxleyi to reveal changes in its protein level and related mechanism.

Original languageEnglish
Article number3028
JournalMolecules
Volume25
Issue number13
DOIs
StatePublished - 2020 Jul

Keywords

  • Emiliania huxleyi
  • Gene ontology
  • Photosynthesis
  • Proteomic profiling
  • Three-dimensional (3D-LC) separation

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