Protein quantification on dendrimer-activated surfaces by using time-of-flight secondary ion mass spectrometry and principal component regression

Young-Pil Kim, Mi Young Hong, Hyun Kyong Shon, Won Chegal, Hyun Mo Cho, Dae Won Moon, Hak Sung Kim, Tae Geol Lee

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Interaction between streptavidin and biotin on poly(amidoamine) (PAMAM) dendrimer-activated surfaces and on self-assembled monolayers (SAMs) was quantitatively studied by using time-of-flight secondary ion mass spectrometry (ToF-SIMS). The surface protein density was systematically varied as a function of protein concentration and independently quantified using the ellipsometry technique. Principal component analysis (PCA) and principal component regression (PCR) were used to identify a correlation between the intensities of the secondary ion peaks and the surface protein densities. From the ToF-SIMS and ellipsometry results, a good linear correlation of protein density was found. Our study shows that surface protein densities are higher on dendrimer-activated surfaces than on SAMs surfaces due to the spherical property of the dendrimer, and that these surface protein densities can be easily quantified with high sensitivity in a label-free manner by ToF-SIMS.

Original languageEnglish
Pages (from-to)1110-1112
Number of pages3
JournalApplied Surface Science
Volume255
Issue number4
DOIs
StatePublished - 2008 Dec 15

Keywords

  • Dendrimer
  • Ellipsometry
  • PCA
  • Quantification
  • SAM
  • Streptavidin
  • ToF-SIMS

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