Preparation and characterization of nonaarginine-modified chitosan nanoparticles for siRNA delivery

Soyeon Park, Eun Ju Jeong, Jangwook Lee, Taiyoun Rhim, Sang Kyung Lee, Kuen Yong Lee

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Chitosan-based nanoparticles have been widely used as a carrier for gene delivery due to their low toxicity and the positively charged amino groups in chitosan. In this study, we hypothesized that introduction of nonaarginine to chitosan could improve its ability to form a complex with siRNA, as well as enhance the cellular uptake and transfection efficiency of chitosan-based nanoparticles. To this end, a peptide with nine repeating arginine residues was chemically coupled to the chitosan backbone, and various characteristics of nonaarginine-chitosan/siRNA nanoparticles were investigated. The mean diameter and zeta potential of the nonaarginine-chitosan/siRNA nanoparticles were dependent on the amount of nonaarginine conjugated to chitosan. Nonaarginine-modified chitosan/siRNA nanoparticles demonstrated enhanced cellular association and transfection efficiency in vitro, while maintaining a low level of cytotoxicity. In conclusion, nonaarginine-modified chitosan should be considered a potential carrier for various gene delivery applications.

Original languageEnglish
Pages (from-to)57-62
Number of pages6
JournalCarbohydrate Polymers
Volume92
Issue number1
DOIs
StatePublished - 2013 Jan 30

Keywords

  • Chitosan
  • Gene delivery
  • Nonaarginine
  • siRNA

Fingerprint Dive into the research topics of 'Preparation and characterization of nonaarginine-modified chitosan nanoparticles for siRNA delivery'. Together they form a unique fingerprint.

  • Cite this