Polymeric gene carriers

Chang Kang Han, Minhyung Lee, Han Bae You

Research output: Contribution to journalReview article

69 Citations (Scopus)

Abstract

Polymeric gene carriers are a potential alternative to using viral vectors. Polymeric carriers have relatively low immunogenicity and cytotoxicity. In addition, polymeric carriers can accommodate large-size DNA, be conjugated with appropriate functionalities, and be administered repeatedly. In spite of these advantages, polymeric gene carriers have some limitations, such as low gene transfection efficiencies and relatively short duration of gene expression. Therefore, extensive research has been conducted toward the development of efficient polymeric carriers. In this review, we discuss current problems associated with polymeric gene carriers and various strategies against transfection barriers - in particular, gene stabilization and protection, cellular targeting, endosomal escaping, nuclear targeting, unpackaging, and biocompatibility. Finally, requirements for future polymeric gene carriers are considered. With all these ongoing efforts, polymeric carriers have become one of the promising gene delivery methods for human gene therapy.

Original languageEnglish
Pages (from-to)317-342
Number of pages26
JournalCritical Reviews in Eukaryotic Gene Expression
Volume15
Issue number4
StatePublished - 2005 Dec 1

Fingerprint

Genes
Transfection
Genetic Therapy
Gene Expression
DNA
Research

Keywords

  • Cellular targeting polyplexes
  • Endosomolytic polyplexes
  • Nonviral gene carriers
  • Nuclear-targeting polyplexes
  • Polymeric gene carriers
  • Stabilized polyplexes

Cite this

Han, C. K., Lee, M., & You, H. B. (2005). Polymeric gene carriers. Critical Reviews in Eukaryotic Gene Expression, 15(4), 317-342.
Han, Chang Kang ; Lee, Minhyung ; You, Han Bae. / Polymeric gene carriers. In: Critical Reviews in Eukaryotic Gene Expression. 2005 ; Vol. 15, No. 4. pp. 317-342.
@article{4a3880620f2f4fff9144becfb7b63200,
title = "Polymeric gene carriers",
abstract = "Polymeric gene carriers are a potential alternative to using viral vectors. Polymeric carriers have relatively low immunogenicity and cytotoxicity. In addition, polymeric carriers can accommodate large-size DNA, be conjugated with appropriate functionalities, and be administered repeatedly. In spite of these advantages, polymeric gene carriers have some limitations, such as low gene transfection efficiencies and relatively short duration of gene expression. Therefore, extensive research has been conducted toward the development of efficient polymeric carriers. In this review, we discuss current problems associated with polymeric gene carriers and various strategies against transfection barriers - in particular, gene stabilization and protection, cellular targeting, endosomal escaping, nuclear targeting, unpackaging, and biocompatibility. Finally, requirements for future polymeric gene carriers are considered. With all these ongoing efforts, polymeric carriers have become one of the promising gene delivery methods for human gene therapy.",
keywords = "Cellular targeting polyplexes, Endosomolytic polyplexes, Nonviral gene carriers, Nuclear-targeting polyplexes, Polymeric gene carriers, Stabilized polyplexes",
author = "Han, {Chang Kang} and Minhyung Lee and You, {Han Bae}",
year = "2005",
month = "12",
day = "1",
language = "English",
volume = "15",
pages = "317--342",
journal = "Critical Reviews in Eukaryotic Gene Expression",
issn = "1045-4403",
number = "4",

}

Han, CK, Lee, M & You, HB 2005, 'Polymeric gene carriers', Critical Reviews in Eukaryotic Gene Expression, vol. 15, no. 4, pp. 317-342.

Polymeric gene carriers. / Han, Chang Kang; Lee, Minhyung; You, Han Bae.

In: Critical Reviews in Eukaryotic Gene Expression, Vol. 15, No. 4, 01.12.2005, p. 317-342.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Polymeric gene carriers

AU - Han, Chang Kang

AU - Lee, Minhyung

AU - You, Han Bae

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Polymeric gene carriers are a potential alternative to using viral vectors. Polymeric carriers have relatively low immunogenicity and cytotoxicity. In addition, polymeric carriers can accommodate large-size DNA, be conjugated with appropriate functionalities, and be administered repeatedly. In spite of these advantages, polymeric gene carriers have some limitations, such as low gene transfection efficiencies and relatively short duration of gene expression. Therefore, extensive research has been conducted toward the development of efficient polymeric carriers. In this review, we discuss current problems associated with polymeric gene carriers and various strategies against transfection barriers - in particular, gene stabilization and protection, cellular targeting, endosomal escaping, nuclear targeting, unpackaging, and biocompatibility. Finally, requirements for future polymeric gene carriers are considered. With all these ongoing efforts, polymeric carriers have become one of the promising gene delivery methods for human gene therapy.

AB - Polymeric gene carriers are a potential alternative to using viral vectors. Polymeric carriers have relatively low immunogenicity and cytotoxicity. In addition, polymeric carriers can accommodate large-size DNA, be conjugated with appropriate functionalities, and be administered repeatedly. In spite of these advantages, polymeric gene carriers have some limitations, such as low gene transfection efficiencies and relatively short duration of gene expression. Therefore, extensive research has been conducted toward the development of efficient polymeric carriers. In this review, we discuss current problems associated with polymeric gene carriers and various strategies against transfection barriers - in particular, gene stabilization and protection, cellular targeting, endosomal escaping, nuclear targeting, unpackaging, and biocompatibility. Finally, requirements for future polymeric gene carriers are considered. With all these ongoing efforts, polymeric carriers have become one of the promising gene delivery methods for human gene therapy.

KW - Cellular targeting polyplexes

KW - Endosomolytic polyplexes

KW - Nonviral gene carriers

KW - Nuclear-targeting polyplexes

KW - Polymeric gene carriers

KW - Stabilized polyplexes

UR - http://www.scopus.com/inward/record.url?scp=33645459721&partnerID=8YFLogxK

M3 - Review article

C2 - 16472063

AN - SCOPUS:33645459721

VL - 15

SP - 317

EP - 342

JO - Critical Reviews in Eukaryotic Gene Expression

JF - Critical Reviews in Eukaryotic Gene Expression

SN - 1045-4403

IS - 4

ER -