Plasma-polymerized antifouling biochips for label-free measurement of protease activity in cell culture media

Jisoo Park, Gae Baik Kim, Andreas Lippitz, Young Mi Kim, Donggeun Jung, Wolfgang E.S. Unger, Young Pil Kim, Tae Geol Lee

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We report polyethylene glycol (PEG)-grafting antifouling surfaces using a plasma copolymerized (PcP) technique to monitor protease activity in complex media. By varying the mixing ratio of the PEG and ethylenediamine (EDA) precursors, the PcP-PEG-EDA (PcP-PE) film was able to easily control surface amine density with good preservation of the internal PEG structure. We found that nonspecific protein adsorption was dramatically reduced in serum-containing media on the PcP-PE films, as opposed to that on plasma polymerized-EDA (PP-E) films without PEG. When SPR sensor chips coated with PcP-PE film were employed to detect protease activity, biotinylated luciferase probes (luciferase-peptide-biotin) on streptavidin-conjugated SPR chips enabled real-time and label-free measurement of matrix metalloproteinase activity in cell culture media. Owing to its excellent antifouling ability, this newly developed method boasts minimal nonspecific binding and can serve as a biochip platform to promote a wide range of applications in the biological field.

Original languageEnglish
Pages (from-to)527-534
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume281
DOIs
StatePublished - 2019 Feb 15

Keywords

  • Antifouling
  • Nonspecific adsorption
  • Plasma copolymerization
  • Protease activity
  • Surface plasmon resonance

Fingerprint Dive into the research topics of 'Plasma-polymerized antifouling biochips for label-free measurement of protease activity in cell culture media'. Together they form a unique fingerprint.

  • Cite this