Phytoremediation of sulfonated Remazol Red dye and textile effluents by Alternanthera philoxeroides: An anatomical, enzymatic and pilot scale study

Niraj R. Rane, Vishal V. Chandanshive, Anuprita D. Watharkar, Rahul V. Khandare, Tejas S. Patil, Pankaj K. Pawar, Sanjay Prabhu Govindwar

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Alternanthera philoxeroides Griseb. a macrophyte was found to degrade a highly sulfonated textile dye Remazol Red (RR) completely within 72h at a concentration of 70mgL-1. An induction in the activities of azoreductase and riboflavin reductase was observed in root and stem tissues; while the activities of lignin peroxidase, laccase and DCIP reductase were induced in leaf tissues. Some enzymes namely tyrosinase, veratryl alcohol oxidase, catalase and superoxide dismutase displayed an increase in their activity in all the tissues in response of 72h exposure to Remazol Red. There was a marginal reduction in contents of chlorophyll a (20%), chlorophyll b (5%) and carotenoids (16%) in the leaves when compared to control plants. A detailed anatomical study of the stem during uptake and treatment revealed a stepwise mechanism of dye degradation. UV-vis spectrophotometric and high performance thin layer chromatographic analyses confirmed the removal of parent dye from solution. Based on the enzymes activities and gas chromatography-mass spectroscopic analysis of degradation products, a possible pathway of phytotransformation of RR was proposed which revealed the formation of 4-(phenylamino)-1,3,5-triazin-2-ol, naphthalene-1-ol and 3-(ethylsulfonyl)phenol. Toxicity study on Devario aequipinnatus fishes showed that the anatomy of gills of fishes exposed to A.philoxeroides treated RR was largely protected. The plants were further explored for rhizofiltration experiments in a pilot scale reactor. A. philoxeroides could decolorize textile industry effluent of varying pH within 96h of treatment which was evident from the significant reductions in the values of American dye manufacturers' institute color, chemical oxygen demand, biological oxygen demand, total dissolved and total suspended solids.

Original languageEnglish
Pages (from-to)271-281
Number of pages11
JournalWater Research
Volume83
DOIs
StatePublished - 2015 Oct 5

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Environmental Biodegradation
Textiles
phytoremediation
Effluents
dye
Coloring Agents
Dyes
effluent
Biological Oxygen Demand Analysis
Chlorophyll
Tissue
Fish
Fishes
stem
Textile Industry
Laccase
Degradation
Spectroscopic analysis
Monophenol Monooxygenase
Riboflavin

Keywords

  • Alternanthera philoxeroides
  • Anatomy
  • Decolorization
  • Enzymes
  • Phytoremediation
  • Rhizofiltration

Cite this

Rane, Niraj R. ; Chandanshive, Vishal V. ; Watharkar, Anuprita D. ; Khandare, Rahul V. ; Patil, Tejas S. ; Pawar, Pankaj K. ; Govindwar, Sanjay Prabhu. / Phytoremediation of sulfonated Remazol Red dye and textile effluents by Alternanthera philoxeroides : An anatomical, enzymatic and pilot scale study. In: Water Research. 2015 ; Vol. 83. pp. 271-281.
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abstract = "Alternanthera philoxeroides Griseb. a macrophyte was found to degrade a highly sulfonated textile dye Remazol Red (RR) completely within 72h at a concentration of 70mgL-1. An induction in the activities of azoreductase and riboflavin reductase was observed in root and stem tissues; while the activities of lignin peroxidase, laccase and DCIP reductase were induced in leaf tissues. Some enzymes namely tyrosinase, veratryl alcohol oxidase, catalase and superoxide dismutase displayed an increase in their activity in all the tissues in response of 72h exposure to Remazol Red. There was a marginal reduction in contents of chlorophyll a (20{\%}), chlorophyll b (5{\%}) and carotenoids (16{\%}) in the leaves when compared to control plants. A detailed anatomical study of the stem during uptake and treatment revealed a stepwise mechanism of dye degradation. UV-vis spectrophotometric and high performance thin layer chromatographic analyses confirmed the removal of parent dye from solution. Based on the enzymes activities and gas chromatography-mass spectroscopic analysis of degradation products, a possible pathway of phytotransformation of RR was proposed which revealed the formation of 4-(phenylamino)-1,3,5-triazin-2-ol, naphthalene-1-ol and 3-(ethylsulfonyl)phenol. Toxicity study on Devario aequipinnatus fishes showed that the anatomy of gills of fishes exposed to A.philoxeroides treated RR was largely protected. The plants were further explored for rhizofiltration experiments in a pilot scale reactor. A. philoxeroides could decolorize textile industry effluent of varying pH within 96h of treatment which was evident from the significant reductions in the values of American dye manufacturers' institute color, chemical oxygen demand, biological oxygen demand, total dissolved and total suspended solids.",
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Phytoremediation of sulfonated Remazol Red dye and textile effluents by Alternanthera philoxeroides : An anatomical, enzymatic and pilot scale study. / Rane, Niraj R.; Chandanshive, Vishal V.; Watharkar, Anuprita D.; Khandare, Rahul V.; Patil, Tejas S.; Pawar, Pankaj K.; Govindwar, Sanjay Prabhu.

In: Water Research, Vol. 83, 05.10.2015, p. 271-281.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Phytoremediation of sulfonated Remazol Red dye and textile effluents by Alternanthera philoxeroides

T2 - An anatomical, enzymatic and pilot scale study

AU - Rane, Niraj R.

AU - Chandanshive, Vishal V.

AU - Watharkar, Anuprita D.

AU - Khandare, Rahul V.

AU - Patil, Tejas S.

AU - Pawar, Pankaj K.

AU - Govindwar, Sanjay Prabhu

PY - 2015/10/5

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