Phosphorylation of ERK and CREB in cultured hippocampal neurons after haloperidol and risperidone administration

Byung Hwan Yang, Hyeon Son, Seok Hyeon Kim, Jung Hyun Nam, Joon Ho Choi, Jun Seok Lee

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The purpose of the present paper was to determine whether the brief exposure of neurons to antipsychotic drugs is associated with the activation of extracellular signal-regulated kinases (ERK) and cyclic adenosine 3′,5′-monophosphate (cAMP) response element (CRE) binding protein (CREB). The activation of ERK-1/2 and CREB can be monitored by immunoblotting with antibodies that specifically recognize p-ERK-1/2 (phosphorylated on Thr-202 and Tyr-204) and p-CREB (phosphorylated on Ser-133). In hippocampal neuron cultures at 25 days in vitro (DIV), the levels of ERK and CREB phosphorylation significantly increased after treatment with haloperidol (50 nmol/L) and risperidone (50 nmol/L), except when risperidone was administered at the p-CREB level. However, risperidone also increased the p-CREB level at an insignificant rate in the same direction. At 10 DIV, none of the antipsychotic drugs significantly increased the level of ERK and CREB phosphorylation. The difference between levels of ERK and CREB phosphorylation in response to haloperidol and risperidone at 25 DIV was also observed. Risperidone significantly increased the level of ERK-1/2 phosphorylation, but not the level of CREB phosphorylation. Haloperidol, in contrast, had a different effect. These data indicate that neuronal maturation affects the phosphorylation of ERK and CREB in response to antipsychotic drugs. Furthermore, these results demonstrate that different antipsychotic drugs could lead to different profiles of ERK and CREB phosphorylation in neurons.

Original languageEnglish
Pages (from-to)262-267
Number of pages6
JournalPsychiatry and Clinical Neurosciences
Volume58
Issue number3
DOIs
StatePublished - 2004 Jun 1

Fingerprint

Risperidone
Extracellular Signal-Regulated MAP Kinases
Haloperidol
Carrier Proteins
Phosphorylation
Neurons
Antipsychotic Agents
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Response Elements
Immunoblotting
Adenosine

Keywords

  • CREB
  • ERK
  • Haloperidol
  • Hippocampal neuron
  • Risperidone

Cite this

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abstract = "The purpose of the present paper was to determine whether the brief exposure of neurons to antipsychotic drugs is associated with the activation of extracellular signal-regulated kinases (ERK) and cyclic adenosine 3′,5′-monophosphate (cAMP) response element (CRE) binding protein (CREB). The activation of ERK-1/2 and CREB can be monitored by immunoblotting with antibodies that specifically recognize p-ERK-1/2 (phosphorylated on Thr-202 and Tyr-204) and p-CREB (phosphorylated on Ser-133). In hippocampal neuron cultures at 25 days in vitro (DIV), the levels of ERK and CREB phosphorylation significantly increased after treatment with haloperidol (50 nmol/L) and risperidone (50 nmol/L), except when risperidone was administered at the p-CREB level. However, risperidone also increased the p-CREB level at an insignificant rate in the same direction. At 10 DIV, none of the antipsychotic drugs significantly increased the level of ERK and CREB phosphorylation. The difference between levels of ERK and CREB phosphorylation in response to haloperidol and risperidone at 25 DIV was also observed. Risperidone significantly increased the level of ERK-1/2 phosphorylation, but not the level of CREB phosphorylation. Haloperidol, in contrast, had a different effect. These data indicate that neuronal maturation affects the phosphorylation of ERK and CREB in response to antipsychotic drugs. Furthermore, these results demonstrate that different antipsychotic drugs could lead to different profiles of ERK and CREB phosphorylation in neurons.",
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Phosphorylation of ERK and CREB in cultured hippocampal neurons after haloperidol and risperidone administration. / Yang, Byung Hwan; Son, Hyeon; Hyeon Kim, Seok; Nam, Jung Hyun; Choi, Joon Ho; Lee, Jun Seok.

In: Psychiatry and Clinical Neurosciences, Vol. 58, No. 3, 01.06.2004, p. 262-267.

Research output: Contribution to journalArticle

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T1 - Phosphorylation of ERK and CREB in cultured hippocampal neurons after haloperidol and risperidone administration

AU - Yang, Byung Hwan

AU - Son, Hyeon

AU - Hyeon Kim, Seok

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AU - Choi, Joon Ho

AU - Lee, Jun Seok

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