Korean Sources metagenome library Korean sources

Translated title of the contribution: Pepper blight disease inhibition metagenome clone screening using soil metagenome library

Hae Chul Park, So Ra Sung, Dong Gwan Kim, Bon Sung Koo, Byeongmoon Jeong, Jinheung Kim, Moon Young Yoon

Research output: Contribution to journalArticle

Abstract

We have purified Phytophthora capsici alpha and beta tubulin from Escherchia coli BL21(DE3). The recombinant alpha and beta tubulins were assembled into microtubule in vitro with specific conditions. The metagenome library was isolated from soil in the Mt. Yeo-Ki, Suwon, Korea and manufactured with the method mentioned in experiment contents for in vitro screening of microtubule assembly screening. FRET effect was used for microtubule assembly inhibitor screening with metagenome library. We got 2 metagenome clones from in vitro screening, and these 2 hit clones showed P. capsici growth inhibition activity on the growing pepper plants. These results suggest that new development of potent inhibitor for pepper blight disease and new approach to prevention of pepper blight disease.

Original languageKorean
Pages (from-to)228-231
Number of pages4
JournalKorean Journal of Microbiology
Volume45
Issue number2
StatePublished - 2009 Jun 1

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Metagenome
Tubulin
Soil
Clone Cells
Microtubules
Phytophthora
Korea
Growth
In Vitro Techniques

Cite this

Park, H. C., Sung, S. R., Kim, D. G., Koo, B. S., Jeong, B., Kim, J., & Yoon, M. Y. (2009). Korean Sources metagenome library Korean sources. Korean Journal of Microbiology, 45(2), 228-231.
Park, Hae Chul ; Sung, So Ra ; Kim, Dong Gwan ; Koo, Bon Sung ; Jeong, Byeongmoon ; Kim, Jinheung ; Yoon, Moon Young. / Korean Sources metagenome library Korean sources. In: Korean Journal of Microbiology. 2009 ; Vol. 45, No. 2. pp. 228-231.
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abstract = "We have purified Phytophthora capsici alpha and beta tubulin from Escherchia coli BL21(DE3). The recombinant alpha and beta tubulins were assembled into microtubule in vitro with specific conditions. The metagenome library was isolated from soil in the Mt. Yeo-Ki, Suwon, Korea and manufactured with the method mentioned in experiment contents for in vitro screening of microtubule assembly screening. FRET effect was used for microtubule assembly inhibitor screening with metagenome library. We got 2 metagenome clones from in vitro screening, and these 2 hit clones showed P. capsici growth inhibition activity on the growing pepper plants. These results suggest that new development of potent inhibitor for pepper blight disease and new approach to prevention of pepper blight disease.",
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Park, HC, Sung, SR, Kim, DG, Koo, BS, Jeong, B, Kim, J & Yoon, MY 2009, 'Korean Sources metagenome library Korean sources', Korean Journal of Microbiology, vol. 45, no. 2, pp. 228-231.

Korean Sources metagenome library Korean sources. / Park, Hae Chul; Sung, So Ra; Kim, Dong Gwan; Koo, Bon Sung; Jeong, Byeongmoon; Kim, Jinheung; Yoon, Moon Young.

In: Korean Journal of Microbiology, Vol. 45, No. 2, 01.06.2009, p. 228-231.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Korean Sources metagenome library Korean sources

AU - Park, Hae Chul

AU - Sung, So Ra

AU - Kim, Dong Gwan

AU - Koo, Bon Sung

AU - Jeong, Byeongmoon

AU - Kim, Jinheung

AU - Yoon, Moon Young

PY - 2009/6/1

Y1 - 2009/6/1

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AB - We have purified Phytophthora capsici alpha and beta tubulin from Escherchia coli BL21(DE3). The recombinant alpha and beta tubulins were assembled into microtubule in vitro with specific conditions. The metagenome library was isolated from soil in the Mt. Yeo-Ki, Suwon, Korea and manufactured with the method mentioned in experiment contents for in vitro screening of microtubule assembly screening. FRET effect was used for microtubule assembly inhibitor screening with metagenome library. We got 2 metagenome clones from in vitro screening, and these 2 hit clones showed P. capsici growth inhibition activity on the growing pepper plants. These results suggest that new development of potent inhibitor for pepper blight disease and new approach to prevention of pepper blight disease.

KW - FRET

KW - Metagenome

KW - Pepper blight disease

KW - Phytophthora capsici

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JO - Korean Journal of Microbiology

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Park HC, Sung SR, Kim DG, Koo BS, Jeong B, Kim J et al. Korean Sources metagenome library Korean sources. Korean Journal of Microbiology. 2009 Jun 1;45(2):228-231.