Pairing of forskolin and KCl increases differentiation of immortalized hippocampal neurons in a CREB Serine 133 phosphorylation-dependent and extracellular-regulated protein kinase-independent manner

Hyeon Son, Kyung Ok Kim, Jin Seuk Kim, Mi Yoon Chang, Sang-Hun Lee, Yong Sung Lee

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Abstract

Although cAMP response element binding protein (CREB)– and extracellular–regulated protein kinase (ERK)–mediated pathways have been linked to each other in neuronal differentiation, involvement of these in hippocampal neuronal cell line has not been defined. Using an immortalized hippocampal cell line, HiB5, we have tried a pairing of forskolin with KCl depolarization, which acts as an ERK and CREB kinase activator in hippocampal neurons, to investigate if an activation of ERK and phosphorylation of CREB at the critical regulatory site, serine 133 might be coupled in differentiation. Differentiation toward a neuronal phenotype was synergistically and markedly increased by the pairing of forskolin and KCl depolarization. The synergistic effect was accompanied by an increase in phosphorylation of CREB Ser-133, but not phosphorylation of ERK, and was not inhibited by MEK inhibitor, PD98059. These findings indicate that phosphorylation of the transcriptional factor CREB may function to facilitate differentiation of HiB5 cells.

Original languageEnglish
Pages (from-to)37-40
Number of pages4
JournalNeuroscience Letters
Volume308
Issue number1
DOIs
StatePublished - 2001 Jul 27

Fingerprint

Cyclic AMP Response Element-Binding Protein
Colforsin
Protein Kinases
Serine
Phosphorylation
Neurons
Cell Line
Mitogen-Activated Protein Kinase Kinases
Cell Differentiation
Phenotype

Keywords

  • Basic fibroblast-derived growth factor
  • CAMP response element binding protein
  • Differentiation
  • Extracellular-regulated protein kinase
  • Forskolin
  • HiB5 cells

Cite this

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title = "Pairing of forskolin and KCl increases differentiation of immortalized hippocampal neurons in a CREB Serine 133 phosphorylation-dependent and extracellular-regulated protein kinase-independent manner",
abstract = "Although cAMP response element binding protein (CREB)– and extracellular–regulated protein kinase (ERK)–mediated pathways have been linked to each other in neuronal differentiation, involvement of these in hippocampal neuronal cell line has not been defined. Using an immortalized hippocampal cell line, HiB5, we have tried a pairing of forskolin with KCl depolarization, which acts as an ERK and CREB kinase activator in hippocampal neurons, to investigate if an activation of ERK and phosphorylation of CREB at the critical regulatory site, serine 133 might be coupled in differentiation. Differentiation toward a neuronal phenotype was synergistically and markedly increased by the pairing of forskolin and KCl depolarization. The synergistic effect was accompanied by an increase in phosphorylation of CREB Ser-133, but not phosphorylation of ERK, and was not inhibited by MEK inhibitor, PD98059. These findings indicate that phosphorylation of the transcriptional factor CREB may function to facilitate differentiation of HiB5 cells.",
keywords = "Basic fibroblast-derived growth factor, CAMP response element binding protein, Differentiation, Extracellular-regulated protein kinase, Forskolin, HiB5 cells",
author = "Hyeon Son and Kim, {Kyung Ok} and Kim, {Jin Seuk} and Chang, {Mi Yoon} and Sang-Hun Lee and Lee, {Yong Sung}",
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T1 - Pairing of forskolin and KCl increases differentiation of immortalized hippocampal neurons in a CREB Serine 133 phosphorylation-dependent and extracellular-regulated protein kinase-independent manner

AU - Son, Hyeon

AU - Kim, Kyung Ok

AU - Kim, Jin Seuk

AU - Chang, Mi Yoon

AU - Lee, Sang-Hun

AU - Lee, Yong Sung

PY - 2001/7/27

Y1 - 2001/7/27

N2 - Although cAMP response element binding protein (CREB)– and extracellular–regulated protein kinase (ERK)–mediated pathways have been linked to each other in neuronal differentiation, involvement of these in hippocampal neuronal cell line has not been defined. Using an immortalized hippocampal cell line, HiB5, we have tried a pairing of forskolin with KCl depolarization, which acts as an ERK and CREB kinase activator in hippocampal neurons, to investigate if an activation of ERK and phosphorylation of CREB at the critical regulatory site, serine 133 might be coupled in differentiation. Differentiation toward a neuronal phenotype was synergistically and markedly increased by the pairing of forskolin and KCl depolarization. The synergistic effect was accompanied by an increase in phosphorylation of CREB Ser-133, but not phosphorylation of ERK, and was not inhibited by MEK inhibitor, PD98059. These findings indicate that phosphorylation of the transcriptional factor CREB may function to facilitate differentiation of HiB5 cells.

AB - Although cAMP response element binding protein (CREB)– and extracellular–regulated protein kinase (ERK)–mediated pathways have been linked to each other in neuronal differentiation, involvement of these in hippocampal neuronal cell line has not been defined. Using an immortalized hippocampal cell line, HiB5, we have tried a pairing of forskolin with KCl depolarization, which acts as an ERK and CREB kinase activator in hippocampal neurons, to investigate if an activation of ERK and phosphorylation of CREB at the critical regulatory site, serine 133 might be coupled in differentiation. Differentiation toward a neuronal phenotype was synergistically and markedly increased by the pairing of forskolin and KCl depolarization. The synergistic effect was accompanied by an increase in phosphorylation of CREB Ser-133, but not phosphorylation of ERK, and was not inhibited by MEK inhibitor, PD98059. These findings indicate that phosphorylation of the transcriptional factor CREB may function to facilitate differentiation of HiB5 cells.

KW - Basic fibroblast-derived growth factor

KW - CAMP response element binding protein

KW - Differentiation

KW - Extracellular-regulated protein kinase

KW - Forskolin

KW - HiB5 cells

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