Optimization of hydrolysis conditions for bovine plasma protein using response surface methodology

Hyun Woo Seo, Eun Young Jung, Gwang Woong Go, Gap Don Kim, Seon Tea Joo, Han Sul Yang

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The purpose of this study was to establish optimal conditions for the hydrolysis of bovine plasma protein. Response surface methodology was used to model and optimize responses [degree of hydrolysis (DH), 2,2-diphenyl-1-picrydrazyl (DPPH) radical-scavenging activity and Fe2+-chelating activity]. Hydrolysis conditions, such as hydrolysis temperature (46.6-63.4 °C), hydrolysis time (98-502 min), and hydrolysis pH (6.32-9.68) were selected as the main processing conditions in the hydrolysis of bovine plasma protein. Optimal conditions for maximum DH (%), DPPH radical-scavenging activity (%) and Fe2+-chelating activity (%) of the hydrolyzed bovine plasma protein, were respectively established. We discovered the following three conditions for optimal hydrolysis of bovine plasma: pH of 7.82-8.32, temperature of 54.1 °C, and time of 338.4-398.4 min. We consequently succeeded in hydrolyzing bovine plasma protein under these conditions and confirmed the various desirable properties of optimal hydrolysis.

Original languageEnglish
Pages (from-to)106-111
Number of pages6
JournalFood Chemistry
Volume185
DOIs
StatePublished - 2015 Oct 15

Keywords

  • DPPH radical scavenging activity
  • Fe<sup>2+</sup> chelating activity
  • Optimization
  • Plasma protein hydrolysates
  • Response surface methodology

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