Nuclear factor-kappa B activation and signal transduction pathway in human intestinal epithelial cells stimulated with Bacteroides fragilis enterotoxin

Jung Mogg Kim, Soo Jin Cho, Shin Jae Kang, Hwoon Yong Jung, Yu Kyoung Oh, Young Jeon Kim, Yang Ja Cho

Research output: Contribution to journalArticle

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Abstract

A ∼20 kDa heat-labile toxin produced by enterotoxigenic Bacteroides fragilis (BFT) induces the expression of CXC chemokines from intestinal epithelial cells. To elucidate signaling pathway involved in chemokine expression of BFT stimulated-epithelial cells, we assessed the activation of nuclear factor-kappa B (NF-κB) by BFT-stimulated human intestinal epithelial cells. The human intestinal epithelial HT-29 cells were incubated with purified BFT. NF-κB signal was detected by electrophoretic mobility shift assay and IκB signals were assessed by immunoblots. Chemokine production was measured by ELISA and mRNA levels were assessed by quantitative RT-PCR. BFT stimulation increased NF-κB signals in HT-29 cells. In contrast, IκBα and IκBε signals were decreased upon BFT stimulation. The subunits involved in the rapid activation of NF-κB were p65 and p50. Transfection of dominant-negative IκBα or IκB kinase (IKK)β plasmid inhibited an up-regulated transcription of downstream target gene (interleukin-8) of NF-κBe. In addition, a blockage of NF-κB activation by MG-132 resulted in down-regulated gene expression of CXC chemokines such as interleukin-8 and growth-related oncogene-α in BFT-stimulated HT-29 cells. These results indicate that NF-κB may function as a central regulator of chemokine gene expression in BFT-stimulated intestinal epithelial cells.

Original languageEnglish
Pages (from-to)343-351
Number of pages9
JournalJournal of Bacteriology and Virology
Volume31
Issue number4
StatePublished - 2001 Jan 1

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NF-kappa B
Signal Transduction
Epithelial Cells
HT29 Cells
Chemokines
CXC Chemokines
Interleukin-8
Gene Expression
Bacteroides fragilis
Electrophoretic Mobility Shift Assay
Regulator Genes
Oncogenes
Transfection
fragilysin
Plasmids
Phosphotransferases
Hot Temperature
Enzyme-Linked Immunosorbent Assay
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Bacteroides fragilis enterotoxin
  • Chemokine
  • NF-κB

Cite this

Kim, Jung Mogg ; Cho, Soo Jin ; Kang, Shin Jae ; Jung, Hwoon Yong ; Oh, Yu Kyoung ; Kim, Young Jeon ; Cho, Yang Ja. / Nuclear factor-kappa B activation and signal transduction pathway in human intestinal epithelial cells stimulated with Bacteroides fragilis enterotoxin. In: Journal of Bacteriology and Virology. 2001 ; Vol. 31, No. 4. pp. 343-351.
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Nuclear factor-kappa B activation and signal transduction pathway in human intestinal epithelial cells stimulated with Bacteroides fragilis enterotoxin. / Kim, Jung Mogg; Cho, Soo Jin; Kang, Shin Jae; Jung, Hwoon Yong; Oh, Yu Kyoung; Kim, Young Jeon; Cho, Yang Ja.

In: Journal of Bacteriology and Virology, Vol. 31, No. 4, 01.01.2001, p. 343-351.

Research output: Contribution to journalArticle

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