New silanization coating for DNA fragment analysis by capillary electrophoresis

Ho Jin Jung, Young Chan Bae

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We propose a new coating technique for fused silica capillaries using silanization with trimethylchlorosilane and diethylamine as a mediating agent in DNA separation using capillary electrophoresis. The proposed coating technique is simple and stable at a high pH. Capillaries coated by the new preparation method give excellent reproducibility for DNA fragment analysis with a good relative standard deviation of less than 0.7% for 150 runs and good stability at pH 8.2.

Original languageEnglish
Pages (from-to)1405-1420
Number of pages16
JournalJournal of Polymer Science, Part A: Polymer Chemistry
Volume40
Issue number10
DOIs
StatePublished - 2002 May 15

Fingerprint

Capillary electrophoresis
Coating techniques
DNA
Coatings
Fused silica
trimethylchlorosilane
diethylamine

Keywords

  • Capillary electrophoresis (CE)
  • Coating techniques
  • Separation of DNA
  • Silanization

Cite this

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New silanization coating for DNA fragment analysis by capillary electrophoresis. / Jung, Ho Jin; Bae, Young Chan.

In: Journal of Polymer Science, Part A: Polymer Chemistry, Vol. 40, No. 10, 15.05.2002, p. 1405-1420.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Jung, Ho Jin

AU - Bae, Young Chan

PY - 2002/5/15

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N2 - We propose a new coating technique for fused silica capillaries using silanization with trimethylchlorosilane and diethylamine as a mediating agent in DNA separation using capillary electrophoresis. The proposed coating technique is simple and stable at a high pH. Capillaries coated by the new preparation method give excellent reproducibility for DNA fragment analysis with a good relative standard deviation of less than 0.7% for 150 runs and good stability at pH 8.2.

AB - We propose a new coating technique for fused silica capillaries using silanization with trimethylchlorosilane and diethylamine as a mediating agent in DNA separation using capillary electrophoresis. The proposed coating technique is simple and stable at a high pH. Capillaries coated by the new preparation method give excellent reproducibility for DNA fragment analysis with a good relative standard deviation of less than 0.7% for 150 runs and good stability at pH 8.2.

KW - Capillary electrophoresis (CE)

KW - Coating techniques

KW - Separation of DNA

KW - Silanization

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