Natural killer cell cytolytic function in Korean patients with adult-onset still's disease

Jeong H.A. Park, Hee Sun Kim, Jin Sook Lee, Jin J. Kim, Kyong Hee Jung, Yong Wook Park, Dae-Hyun Yoo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective. To investigate natural killer (NK) cell proportions, NK cell cytotoxicity, and interleukin 18 (IL-18) expression, in patients with adult-onset Still's disease (AOSD). Methods. Forty-five patients with AOSD (active = 22, inactive = 23) and 32 healthy controls were included. The proportions of NK cells among peripheral blood mononuclear cells were assessed by flow cytometry. IL-18 and IL-18-binding protein (IL-18BP) concentrations were measured by ELISA. Twenty-four patients with AOSD and 18 controls were examined for cytotoxic activity of NK cells by co-incubating NK cells with NK-sensitive K562 cells. The association of NK cell function with clinical and laboratory measures was investigated. Results. The proportions of NK cells were significantly lower in patients with active AOSD than in patients with inactive disease and controls. NK cell cytotoxic function was significantly lower in patients with AOSD than in controls. NK cell proportions and cytotoxic functions were reexamined in 11 and 6 patients, respectively, after treatment. Low NK cell proportion and cytotoxic dysfunction were improved with clinical improvements of the patients. IL-18 and IL-18BP levels were much higher in patients with active AOSD than in controls. NK cell cytotoxic functions were consistently low and IL-18 and IL-18BP levels were constantly high in patients with AOSD, regardless of disease activity. Conclusion. Low NK cell proportion, defective cytotoxic function, and elevated IL-18 levels may be significant features of AOSD. After resolution of the acute phase, low NK cell proportion was recovered and NK cell cytolytic function was restored along with clinical improvement. These findings possibly contribute to immunologic abnormalities in AOSD. The Journal of Rheumatology

Original languageEnglish
Pages (from-to)2000-2007
Number of pages8
JournalJournal of Rheumatology
Volume39
Issue number10
DOIs
StatePublished - 2012 Oct 1

Fingerprint

Adult-Onset Still's Disease
Natural Killer Cells
Interleukin-18
K562 Cells
Rheumatology

Keywords

  • Adult-onset still's disease
  • Interleukin 18
  • Interleukin 18 binding protein
  • Macrophage activation syndrome
  • Natural killer cell

Cite this

Park, Jeong H.A. ; Kim, Hee Sun ; Lee, Jin Sook ; Kim, Jin J. ; Jung, Kyong Hee ; Park, Yong Wook ; Yoo, Dae-Hyun. / Natural killer cell cytolytic function in Korean patients with adult-onset still's disease. In: Journal of Rheumatology. 2012 ; Vol. 39, No. 10. pp. 2000-2007.
@article{686262235b3f4e5fa73cd58884f62eb1,
title = "Natural killer cell cytolytic function in Korean patients with adult-onset still's disease",
abstract = "Objective. To investigate natural killer (NK) cell proportions, NK cell cytotoxicity, and interleukin 18 (IL-18) expression, in patients with adult-onset Still's disease (AOSD). Methods. Forty-five patients with AOSD (active = 22, inactive = 23) and 32 healthy controls were included. The proportions of NK cells among peripheral blood mononuclear cells were assessed by flow cytometry. IL-18 and IL-18-binding protein (IL-18BP) concentrations were measured by ELISA. Twenty-four patients with AOSD and 18 controls were examined for cytotoxic activity of NK cells by co-incubating NK cells with NK-sensitive K562 cells. The association of NK cell function with clinical and laboratory measures was investigated. Results. The proportions of NK cells were significantly lower in patients with active AOSD than in patients with inactive disease and controls. NK cell cytotoxic function was significantly lower in patients with AOSD than in controls. NK cell proportions and cytotoxic functions were reexamined in 11 and 6 patients, respectively, after treatment. Low NK cell proportion and cytotoxic dysfunction were improved with clinical improvements of the patients. IL-18 and IL-18BP levels were much higher in patients with active AOSD than in controls. NK cell cytotoxic functions were consistently low and IL-18 and IL-18BP levels were constantly high in patients with AOSD, regardless of disease activity. Conclusion. Low NK cell proportion, defective cytotoxic function, and elevated IL-18 levels may be significant features of AOSD. After resolution of the acute phase, low NK cell proportion was recovered and NK cell cytolytic function was restored along with clinical improvement. These findings possibly contribute to immunologic abnormalities in AOSD. The Journal of Rheumatology",
keywords = "Adult-onset still's disease, Interleukin 18, Interleukin 18 binding protein, Macrophage activation syndrome, Natural killer cell",
author = "Park, {Jeong H.A.} and Kim, {Hee Sun} and Lee, {Jin Sook} and Kim, {Jin J.} and Jung, {Kyong Hee} and Park, {Yong Wook} and Dae-Hyun Yoo",
year = "2012",
month = "10",
day = "1",
doi = "10.3899/jrheum.111500",
language = "English",
volume = "39",
pages = "2000--2007",
journal = "Journal of Rheumatology",
issn = "0315-162X",
number = "10",

}

Natural killer cell cytolytic function in Korean patients with adult-onset still's disease. / Park, Jeong H.A.; Kim, Hee Sun; Lee, Jin Sook; Kim, Jin J.; Jung, Kyong Hee; Park, Yong Wook; Yoo, Dae-Hyun.

In: Journal of Rheumatology, Vol. 39, No. 10, 01.10.2012, p. 2000-2007.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Natural killer cell cytolytic function in Korean patients with adult-onset still's disease

AU - Park, Jeong H.A.

AU - Kim, Hee Sun

AU - Lee, Jin Sook

AU - Kim, Jin J.

AU - Jung, Kyong Hee

AU - Park, Yong Wook

AU - Yoo, Dae-Hyun

PY - 2012/10/1

Y1 - 2012/10/1

N2 - Objective. To investigate natural killer (NK) cell proportions, NK cell cytotoxicity, and interleukin 18 (IL-18) expression, in patients with adult-onset Still's disease (AOSD). Methods. Forty-five patients with AOSD (active = 22, inactive = 23) and 32 healthy controls were included. The proportions of NK cells among peripheral blood mononuclear cells were assessed by flow cytometry. IL-18 and IL-18-binding protein (IL-18BP) concentrations were measured by ELISA. Twenty-four patients with AOSD and 18 controls were examined for cytotoxic activity of NK cells by co-incubating NK cells with NK-sensitive K562 cells. The association of NK cell function with clinical and laboratory measures was investigated. Results. The proportions of NK cells were significantly lower in patients with active AOSD than in patients with inactive disease and controls. NK cell cytotoxic function was significantly lower in patients with AOSD than in controls. NK cell proportions and cytotoxic functions were reexamined in 11 and 6 patients, respectively, after treatment. Low NK cell proportion and cytotoxic dysfunction were improved with clinical improvements of the patients. IL-18 and IL-18BP levels were much higher in patients with active AOSD than in controls. NK cell cytotoxic functions were consistently low and IL-18 and IL-18BP levels were constantly high in patients with AOSD, regardless of disease activity. Conclusion. Low NK cell proportion, defective cytotoxic function, and elevated IL-18 levels may be significant features of AOSD. After resolution of the acute phase, low NK cell proportion was recovered and NK cell cytolytic function was restored along with clinical improvement. These findings possibly contribute to immunologic abnormalities in AOSD. The Journal of Rheumatology

AB - Objective. To investigate natural killer (NK) cell proportions, NK cell cytotoxicity, and interleukin 18 (IL-18) expression, in patients with adult-onset Still's disease (AOSD). Methods. Forty-five patients with AOSD (active = 22, inactive = 23) and 32 healthy controls were included. The proportions of NK cells among peripheral blood mononuclear cells were assessed by flow cytometry. IL-18 and IL-18-binding protein (IL-18BP) concentrations were measured by ELISA. Twenty-four patients with AOSD and 18 controls were examined for cytotoxic activity of NK cells by co-incubating NK cells with NK-sensitive K562 cells. The association of NK cell function with clinical and laboratory measures was investigated. Results. The proportions of NK cells were significantly lower in patients with active AOSD than in patients with inactive disease and controls. NK cell cytotoxic function was significantly lower in patients with AOSD than in controls. NK cell proportions and cytotoxic functions were reexamined in 11 and 6 patients, respectively, after treatment. Low NK cell proportion and cytotoxic dysfunction were improved with clinical improvements of the patients. IL-18 and IL-18BP levels were much higher in patients with active AOSD than in controls. NK cell cytotoxic functions were consistently low and IL-18 and IL-18BP levels were constantly high in patients with AOSD, regardless of disease activity. Conclusion. Low NK cell proportion, defective cytotoxic function, and elevated IL-18 levels may be significant features of AOSD. After resolution of the acute phase, low NK cell proportion was recovered and NK cell cytolytic function was restored along with clinical improvement. These findings possibly contribute to immunologic abnormalities in AOSD. The Journal of Rheumatology

KW - Adult-onset still's disease

KW - Interleukin 18

KW - Interleukin 18 binding protein

KW - Macrophage activation syndrome

KW - Natural killer cell

UR - http://www.scopus.com/inward/record.url?scp=84867051755&partnerID=8YFLogxK

U2 - 10.3899/jrheum.111500

DO - 10.3899/jrheum.111500

M3 - Article

C2 - 22859348

AN - SCOPUS:84867051755

VL - 39

SP - 2000

EP - 2007

JO - Journal of Rheumatology

JF - Journal of Rheumatology

SN - 0315-162X

IS - 10

ER -