MUC expression in gallbladder epithelial tissues in cholesterol-associated gallbladder disease

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Abstract

Background/Aims: Gallstone pathogenesis is linked to mucin hypersecretion and bacterial infection. Several mucin genes have been identified in gallbladder epithelial cells (GBECs). We investigated MUC expression in cholesterolassociated gallbladder disease and evaluated the relationship between mucin and bacterial infection. Methods: The present study involved 20 patients with cholesterol stones with cholecystitis, five with cholesterol stones with cholesterolosis, six with cholesterol polyps, two with gallbladder cancer, and six controls. Canine GBECs treated with lipopolysaccharide were also studied. MUC3, MUC5AC, MUC5B, and MUC6 antibodies were used for dot/slot immunoblotting and immunohistochemical studies of the gallbladder epithelial tissues, canine GBECs, and bile. Reverse-transcription polymerase chain reaction was performed to evaluate MUC3 and MUC5B expression. Results: MUC3, MUC5AC, MUC5B, and MUC6 were expressed in the normal gallbladder epithelium, and of those, MUC3 and MUC5B exhibited the highest expression levels. Greatly increased levels of MUC3 and MUC5B expression were observed in the cholesterol stone group, and slightly increased levels were observed in the cholesterol polyp group; MUC3 and MUC5B mRNA was also upregulated in those groups. Canine GBECs treated with lipopolysaccharide also showed upregulation of MUC3 and MUC5B. Conclusions: The mucin genes with the highest expression levels in gallbladder tissue in cholesterol-associated diseases were MUC3 and MUC5B. Cholesterol stones and gallbladder infections were associated with increased MUC3 and MUC5B expression.

Original languageEnglish
Pages (from-to)851-858
Number of pages8
JournalGut and Liver
Volume10
Issue number5
DOIs
StatePublished - 2016 Sep

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Gallbladder Diseases
Gallbladder
Epithelium
Cholesterol
Mucins
Epithelial Cells
Canidae
Polyps
Bacterial Infections
Lipopolysaccharides
Gallbladder Neoplasms
Cholecystitis
Gallstones
Immunoblotting
Bile
Genes
Reverse Transcription
Up-Regulation
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Cholesterol
  • Gallstones
  • Infection
  • MUC3
  • MUC5B

Cite this

@article{9b5976169032446b8223d55b5b151393,
title = "MUC expression in gallbladder epithelial tissues in cholesterol-associated gallbladder disease",
abstract = "Background/Aims: Gallstone pathogenesis is linked to mucin hypersecretion and bacterial infection. Several mucin genes have been identified in gallbladder epithelial cells (GBECs). We investigated MUC expression in cholesterolassociated gallbladder disease and evaluated the relationship between mucin and bacterial infection. Methods: The present study involved 20 patients with cholesterol stones with cholecystitis, five with cholesterol stones with cholesterolosis, six with cholesterol polyps, two with gallbladder cancer, and six controls. Canine GBECs treated with lipopolysaccharide were also studied. MUC3, MUC5AC, MUC5B, and MUC6 antibodies were used for dot/slot immunoblotting and immunohistochemical studies of the gallbladder epithelial tissues, canine GBECs, and bile. Reverse-transcription polymerase chain reaction was performed to evaluate MUC3 and MUC5B expression. Results: MUC3, MUC5AC, MUC5B, and MUC6 were expressed in the normal gallbladder epithelium, and of those, MUC3 and MUC5B exhibited the highest expression levels. Greatly increased levels of MUC3 and MUC5B expression were observed in the cholesterol stone group, and slightly increased levels were observed in the cholesterol polyp group; MUC3 and MUC5B mRNA was also upregulated in those groups. Canine GBECs treated with lipopolysaccharide also showed upregulation of MUC3 and MUC5B. Conclusions: The mucin genes with the highest expression levels in gallbladder tissue in cholesterol-associated diseases were MUC3 and MUC5B. Cholesterol stones and gallbladder infections were associated with increased MUC3 and MUC5B expression.",
keywords = "Cholesterol, Gallstones, Infection, MUC3, MUC5B",
author = "Yoo, {Kyo Sang} and Choi, {Ho Soon} and Jun, {Dae Won} and Lee, {Hang Lak} and Lee, {Oh Young} and Yoon, {Byung Chul} and Lee, {Kyeong Geun} and Paik, {Seung Sam} and Kim, {Yong Seok} and Jin Lee",
year = "2016",
month = "9",
doi = "10.5009/gnl15600",
language = "English",
volume = "10",
pages = "851--858",
journal = "Gut and liver",
issn = "1976-2283",
number = "5",

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TY - JOUR

T1 - MUC expression in gallbladder epithelial tissues in cholesterol-associated gallbladder disease

AU - Yoo, Kyo Sang

AU - Choi, Ho Soon

AU - Jun, Dae Won

AU - Lee, Hang Lak

AU - Lee, Oh Young

AU - Yoon, Byung Chul

AU - Lee, Kyeong Geun

AU - Paik, Seung Sam

AU - Kim, Yong Seok

AU - Lee, Jin

PY - 2016/9

Y1 - 2016/9

N2 - Background/Aims: Gallstone pathogenesis is linked to mucin hypersecretion and bacterial infection. Several mucin genes have been identified in gallbladder epithelial cells (GBECs). We investigated MUC expression in cholesterolassociated gallbladder disease and evaluated the relationship between mucin and bacterial infection. Methods: The present study involved 20 patients with cholesterol stones with cholecystitis, five with cholesterol stones with cholesterolosis, six with cholesterol polyps, two with gallbladder cancer, and six controls. Canine GBECs treated with lipopolysaccharide were also studied. MUC3, MUC5AC, MUC5B, and MUC6 antibodies were used for dot/slot immunoblotting and immunohistochemical studies of the gallbladder epithelial tissues, canine GBECs, and bile. Reverse-transcription polymerase chain reaction was performed to evaluate MUC3 and MUC5B expression. Results: MUC3, MUC5AC, MUC5B, and MUC6 were expressed in the normal gallbladder epithelium, and of those, MUC3 and MUC5B exhibited the highest expression levels. Greatly increased levels of MUC3 and MUC5B expression were observed in the cholesterol stone group, and slightly increased levels were observed in the cholesterol polyp group; MUC3 and MUC5B mRNA was also upregulated in those groups. Canine GBECs treated with lipopolysaccharide also showed upregulation of MUC3 and MUC5B. Conclusions: The mucin genes with the highest expression levels in gallbladder tissue in cholesterol-associated diseases were MUC3 and MUC5B. Cholesterol stones and gallbladder infections were associated with increased MUC3 and MUC5B expression.

AB - Background/Aims: Gallstone pathogenesis is linked to mucin hypersecretion and bacterial infection. Several mucin genes have been identified in gallbladder epithelial cells (GBECs). We investigated MUC expression in cholesterolassociated gallbladder disease and evaluated the relationship between mucin and bacterial infection. Methods: The present study involved 20 patients with cholesterol stones with cholecystitis, five with cholesterol stones with cholesterolosis, six with cholesterol polyps, two with gallbladder cancer, and six controls. Canine GBECs treated with lipopolysaccharide were also studied. MUC3, MUC5AC, MUC5B, and MUC6 antibodies were used for dot/slot immunoblotting and immunohistochemical studies of the gallbladder epithelial tissues, canine GBECs, and bile. Reverse-transcription polymerase chain reaction was performed to evaluate MUC3 and MUC5B expression. Results: MUC3, MUC5AC, MUC5B, and MUC6 were expressed in the normal gallbladder epithelium, and of those, MUC3 and MUC5B exhibited the highest expression levels. Greatly increased levels of MUC3 and MUC5B expression were observed in the cholesterol stone group, and slightly increased levels were observed in the cholesterol polyp group; MUC3 and MUC5B mRNA was also upregulated in those groups. Canine GBECs treated with lipopolysaccharide also showed upregulation of MUC3 and MUC5B. Conclusions: The mucin genes with the highest expression levels in gallbladder tissue in cholesterol-associated diseases were MUC3 and MUC5B. Cholesterol stones and gallbladder infections were associated with increased MUC3 and MUC5B expression.

KW - Cholesterol

KW - Gallstones

KW - Infection

KW - MUC3

KW - MUC5B

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U2 - 10.5009/gnl15600

DO - 10.5009/gnl15600

M3 - Article

C2 - 27563024

AN - SCOPUS:84990066393

VL - 10

SP - 851

EP - 858

JO - Gut and liver

JF - Gut and liver

SN - 1976-2283

IS - 5

ER -