Molecular cloning and characterization of bovine bgl-1, a novel family member of WD-40 repeat-containing lethal giant larvae tumor suppressor genes.

Kwang Hyun Baek, Yong Soo Kim, Sunki Jung, Kwang Youl Lee, Hee Kyung Choi, Kye Seong Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In this study, we have isolated a bovine homologue bgl-1 of lethal giant larvae (lgl) tumor suppressor oncogene from bovine brain by RT-PCR using primers designed based on the conserved sequences for lgl family members. The sequence analysis showed that the bgl-1 encodes a 1,036 amino acid polypeptide with the molecular weight of approximately 112 kDa containing a domain characteristic of WD-40 proteins. The amino acid sequence of bgl-1 showed a homology of 98.3 and 87.3% identity to that of mouse and human, respectively. Northern blot analysis showed that bgl-1 was highly expressed in brain, ovary and testis, with moderate expression in liver, uterus, lung and kidney. This suggests that the bgl-1 may play essential roles in each of these organs. The complementation analysis revealed that the bovine bgl-1 partially restored the Na+ tolerance in the absence of yeast lgl homologue, suggesting that bgl-1 is a bovine homologue of the lgl family.

Original languageEnglish
Pages (from-to)739-744
Number of pages6
JournalInternational Journal of Oncology
Volume20
Issue number4
StatePublished - 2002 Apr

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Molecular Cloning
Tumor Suppressor Genes
Larva
Conserved Sequence
Brain
Oncogenes
Northern Blotting
Uterus
Sequence Analysis
Testis
Amino Acid Sequence
Ovary
Yeasts
Molecular Weight
Kidney
Amino Acids
Polymerase Chain Reaction
Lung
Peptides
WD40 Repeats

Cite this

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title = "Molecular cloning and characterization of bovine bgl-1, a novel family member of WD-40 repeat-containing lethal giant larvae tumor suppressor genes.",
abstract = "In this study, we have isolated a bovine homologue bgl-1 of lethal giant larvae (lgl) tumor suppressor oncogene from bovine brain by RT-PCR using primers designed based on the conserved sequences for lgl family members. The sequence analysis showed that the bgl-1 encodes a 1,036 amino acid polypeptide with the molecular weight of approximately 112 kDa containing a domain characteristic of WD-40 proteins. The amino acid sequence of bgl-1 showed a homology of 98.3 and 87.3{\%} identity to that of mouse and human, respectively. Northern blot analysis showed that bgl-1 was highly expressed in brain, ovary and testis, with moderate expression in liver, uterus, lung and kidney. This suggests that the bgl-1 may play essential roles in each of these organs. The complementation analysis revealed that the bovine bgl-1 partially restored the Na+ tolerance in the absence of yeast lgl homologue, suggesting that bgl-1 is a bovine homologue of the lgl family.",
author = "Baek, {Kwang Hyun} and Kim, {Yong Soo} and Sunki Jung and Lee, {Kwang Youl} and Choi, {Hee Kyung} and Kim, {Kye Seong}",
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Molecular cloning and characterization of bovine bgl-1, a novel family member of WD-40 repeat-containing lethal giant larvae tumor suppressor genes. / Baek, Kwang Hyun; Kim, Yong Soo; Jung, Sunki; Lee, Kwang Youl; Choi, Hee Kyung; Kim, Kye Seong.

In: International Journal of Oncology, Vol. 20, No. 4, 04.2002, p. 739-744.

Research output: Contribution to journalArticle

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AU - Choi, Hee Kyung

AU - Kim, Kye Seong

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