Microfluidic image cytometry (μFIC) assessments of silver nanoparticle cytotoxicity

Jonghoon Park, Tae Hyun Yoon

Research output: Contribution to journalArticle

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Abstract

Cytotoxicity assessment of silver nanoparticles (AgNPs) was performed using MTT-based microfluidic image cytometry (μFIC). The LC50 value of HeLa cells exposed to AgNPs in the microfluidic device was estimated as 46.7 mg/L, which is similar to that estimated by MTT-based IC for cells cultured in a 96 well plate (49.9 mg/ L). These results confirm that the μFIC approach can produce cytotoxicity data that is reasonably well-matched with that of the conventional 96 well plate system with much higher efficiency. This μFIC method provides many benefits including ease of use and low cost, and is a more rapid in vitro cell based assay for AgNPs. This may aid in speeding up data acquisition in the field of nanosafety and make a significant contribution to the quantitative understanding of nanoproperty- toxicity relationships.

Original languageEnglish
Pages (from-to)4023-4027
Number of pages5
JournalBulletin of the Korean Chemical Society
Volume33
Issue number12
DOIs
Publication statusPublished - 2012 Dec 20

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Keywords

  • Ag nanoparticles
  • Cytotoxicity assessment
  • Microfluidic device
  • Microfluidic image cytometry
  • Nanotoxicity

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