Microbial community acclimatization for enhancement in the methane productivity of anaerobic co-digestion of fats, oil, and grease

Mayur B. Kurade, Shouvik Saha, Jung Rae Kim, Hyun Seog Roh, Byong Hun Jeon

Research output: Contribution to journalArticle

Abstract

The methane productivity and long chain fatty acids (LCFAs) degradation capability of unacclimatized seed sludge (USS) and acclimatized seed sludge (ASS) at different substrate ratios of fats oil and grease (FOG) and mixed sewage sludge were investigated in this study. Biogas produced in ASS in initial phase of anaerobic digestion had higher methane content (65–76%) than that in USS (26–73%). The degradation of major LCFAs in the ASS was 22–80%, 33–191%, and 7–64% higher for the substrate ratios of 100:10, 100:20, and 100:30, respectively, as compared to the LCFAs’ degradation in USS. Microbial acclimatization increased the population of Firmicutes (40%), Bacteroidetes (32%), Synergistetes (10%), and Euryarchaeota (8%) in ASS, which supported the faster rate of LCFAs degradation for its later conversion to methane. The significant abundance of Syntrophomonas and Methanosarcina genera in ASS supported faster generation rate of methane in an obligatory syntrophic relationship.

Original languageEnglish
Article number122294
JournalBioresource Technology
Volume296
DOIs
StatePublished - 2020 Jan

Fingerprint

Methane
Lubricating greases
Oils and fats
acclimation
fat
Seed
digestion
microbial community
Productivity
methane
sludge
seed
productivity
oil
Fatty acids
Fatty Acids
fatty acid
Degradation
degradation
substrate

Keywords

  • Acclimation
  • Anaerobic co-digestion
  • Biomethane
  • Fats, oil, and grease
  • High-throughput sequencing
  • Long-chain fatty acids

Cite this

@article{1e8a36f5f5bd4ddea5be81662528be08,
title = "Microbial community acclimatization for enhancement in the methane productivity of anaerobic co-digestion of fats, oil, and grease",
abstract = "The methane productivity and long chain fatty acids (LCFAs) degradation capability of unacclimatized seed sludge (USS) and acclimatized seed sludge (ASS) at different substrate ratios of fats oil and grease (FOG) and mixed sewage sludge were investigated in this study. Biogas produced in ASS in initial phase of anaerobic digestion had higher methane content (65–76{\%}) than that in USS (26–73{\%}). The degradation of major LCFAs in the ASS was 22–80{\%}, 33–191{\%}, and 7–64{\%} higher for the substrate ratios of 100:10, 100:20, and 100:30, respectively, as compared to the LCFAs’ degradation in USS. Microbial acclimatization increased the population of Firmicutes (40{\%}), Bacteroidetes (32{\%}), Synergistetes (10{\%}), and Euryarchaeota (8{\%}) in ASS, which supported the faster rate of LCFAs degradation for its later conversion to methane. The significant abundance of Syntrophomonas and Methanosarcina genera in ASS supported faster generation rate of methane in an obligatory syntrophic relationship.",
keywords = "Acclimation, Anaerobic co-digestion, Biomethane, Fats, oil, and grease, High-throughput sequencing, Long-chain fatty acids",
author = "Kurade, {Mayur B.} and Shouvik Saha and Kim, {Jung Rae} and Roh, {Hyun Seog} and Jeon, {Byong Hun}",
year = "2020",
month = "1",
doi = "10.1016/j.biortech.2019.122294",
language = "English",
volume = "296",
journal = "Bioresource Technology",
issn = "0960-8524",

}

Microbial community acclimatization for enhancement in the methane productivity of anaerobic co-digestion of fats, oil, and grease. / Kurade, Mayur B.; Saha, Shouvik; Kim, Jung Rae; Roh, Hyun Seog; Jeon, Byong Hun.

In: Bioresource Technology, Vol. 296, 122294, 01.2020.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Microbial community acclimatization for enhancement in the methane productivity of anaerobic co-digestion of fats, oil, and grease

AU - Kurade, Mayur B.

AU - Saha, Shouvik

AU - Kim, Jung Rae

AU - Roh, Hyun Seog

AU - Jeon, Byong Hun

PY - 2020/1

Y1 - 2020/1

N2 - The methane productivity and long chain fatty acids (LCFAs) degradation capability of unacclimatized seed sludge (USS) and acclimatized seed sludge (ASS) at different substrate ratios of fats oil and grease (FOG) and mixed sewage sludge were investigated in this study. Biogas produced in ASS in initial phase of anaerobic digestion had higher methane content (65–76%) than that in USS (26–73%). The degradation of major LCFAs in the ASS was 22–80%, 33–191%, and 7–64% higher for the substrate ratios of 100:10, 100:20, and 100:30, respectively, as compared to the LCFAs’ degradation in USS. Microbial acclimatization increased the population of Firmicutes (40%), Bacteroidetes (32%), Synergistetes (10%), and Euryarchaeota (8%) in ASS, which supported the faster rate of LCFAs degradation for its later conversion to methane. The significant abundance of Syntrophomonas and Methanosarcina genera in ASS supported faster generation rate of methane in an obligatory syntrophic relationship.

AB - The methane productivity and long chain fatty acids (LCFAs) degradation capability of unacclimatized seed sludge (USS) and acclimatized seed sludge (ASS) at different substrate ratios of fats oil and grease (FOG) and mixed sewage sludge were investigated in this study. Biogas produced in ASS in initial phase of anaerobic digestion had higher methane content (65–76%) than that in USS (26–73%). The degradation of major LCFAs in the ASS was 22–80%, 33–191%, and 7–64% higher for the substrate ratios of 100:10, 100:20, and 100:30, respectively, as compared to the LCFAs’ degradation in USS. Microbial acclimatization increased the population of Firmicutes (40%), Bacteroidetes (32%), Synergistetes (10%), and Euryarchaeota (8%) in ASS, which supported the faster rate of LCFAs degradation for its later conversion to methane. The significant abundance of Syntrophomonas and Methanosarcina genera in ASS supported faster generation rate of methane in an obligatory syntrophic relationship.

KW - Acclimation

KW - Anaerobic co-digestion

KW - Biomethane

KW - Fats, oil, and grease

KW - High-throughput sequencing

KW - Long-chain fatty acids

UR - http://www.scopus.com/inward/record.url?scp=85074169683&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2019.122294

DO - 10.1016/j.biortech.2019.122294

M3 - Article

C2 - 31677410

AN - SCOPUS:85074169683

VL - 296

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

M1 - 122294

ER -