Low expression of single-stranded DNA binding protein 2 (SSBP2) predicts unfavourable postoperative outcomes in patients with clear cell renal cell carcinoma

Hyunsung Kim, Yeseul Kim, Seongsik Bang, Seongeon Park, Seungyun Jee, Jongmin Sim, Su Jin Shin, Seung Sam Paik, Kiseok Jang

Research output: Contribution to journalArticle

Abstract

Background: Single-stranded DNA binding protein 2 (SSBP2) is a subunit of a single-stranded DNA binding complex, which is involved in the maintenance of hematopoietic stem cells and stress responses. Numerous studies have suggested that SSBP2 functions as a tumor suppressor and is silenced through a pathway mediated by promoter hypermethylation. However, the role of SSBP2 in human renal cell carcinoma has not been reported, to date. Herein, we investigated the clinicopathological significance of SSBP2 expression in clear cell renal cell carcinoma (ccRCC). Materials and Methods: We constructed tissue microarrays consisting of 173 ccRCC tissues, and SSBP2 expression was evaluated semi-quantitatively based on the staining intensity and the proportion of stained cells. Regarding statistical analysis, the tissues were divided into two groups according to SSBP2 expression, and correlation of SSBP2 expression with various clinicopathological characteristics and patient outcomes was evaluated. Results: Low SSBP2 expression was observed in 114 of 175 (65.9%) of ccRCC cases, and low SSBP2 expression was significantly correlated with larger tumor size (p=0.005, Chi-square test), higher WHO/ISUP histological grade (p<0.001, Chi-square test), tumor necrosis (p=0.008, Chi-square test), sarcomatoid change (p=0.021, Chi-square test), and higher pT AJCC stage (p=0.002, Chi-square test). Kaplan-Meier survival curves revealed that patients with low SSBP2 expression had worse recurrence-free survival (p=0.041, log-rank test). Conclusion: ccRCC with low SSBP2 expression was associated with adverse clinicopathological characteristics and poor patient outcomes.

Original languageEnglish
Pages (from-to)101-107
Number of pages7
JournalIn Vivo
Volume34
Issue number1
DOIs
StatePublished - 2020 Jan 1

Keywords

  • Cancer prognosis
  • Clear cell renal cell carcinoma
  • Immunohistochemistry
  • Single-stranded DNA binding protein 2

Fingerprint Dive into the research topics of 'Low expression of single-stranded DNA binding protein 2 (SSBP2) predicts unfavourable postoperative outcomes in patients with clear cell renal cell carcinoma'. Together they form a unique fingerprint.

  • Cite this