Low-dose γ-irradiation induces dual radio-adaptive responses depending on the post-irradiation time by altering microRNA expression profiles in normal human dermal fibroblasts

Seunghee Bae, Karam Kim, Hwa Jun Cha, Yeongmin Choi, Shang Hun Shin, In Sook An, Jae Ho Lee, Su Jae Lee, Ji Young Kim, Seon Young Nam, Sungkwan An

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Exposure to high-dose ionizing radiation, including γ-radiation, induces severe skin disorders. However, the biological consequences and molecular mechanisms responsible for the response of human skin to low-dose γ-radiation (LDR) are largely unknown. In the present study, we demonstrate that LDR (0.1 Gy) induces distinct cellular responses in normal human dermal fibroblasts (NHDFs) depending on the post-irradiation time point. A MTT-based cell viability assay and propidium iodide staining-based cell cycle assay revealed that the viability and proportion of the cells in the G2/M phase were differed at 6 and 24 h post-irradiation. Reverse transcription quantitative PCR (RT-qPCR) revealed that LDR significantly upregulated the mRNA expression of collagen type I alpha 1 (COL1A1), but downregulated the mRNA expression of matrix metalloproteinase 1 (MMP1) at 24 h post-irradiation. MicroRNA (miRNA) microarray analysis further demonstrated that LDR induced changes in the expression profiles of specific miRNAs and that some of the deregulated miRNAs were specific to either the early or late radio-adaptive response. Our results suggest that LDR generates dual radio-adaptive responses depending on the post-irradiation time by altering specific miRNA expression profiles in NHDFs.

Original languageEnglish
Pages (from-to)227-237
Number of pages11
JournalInternational Journal of Molecular Medicine
Volume35
Issue number1
DOIs
StatePublished - 2015 Jan 1

Keywords

  • Dermal fibroblasts
  • Expression
  • Low-dose radiation
  • MicroRNA
  • Radio-adaptive response

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