Loss of CpSRP54 function leads to a truncated light-harvesting antenna size in Chlamydomonas reinhardtii

Jooyeon Jeong, Kwangryul Baek, Henning Kirst, Anastasios Melis, EonSeon Jin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The Chlamydomonas reinhardtii truncated light-harvesting antenna 4 (tla4) DNA transposon mutant has a pale green phenotype, a lower chlorophyll (Chl) per cell and a higher Chl a/b ratio in comparison with the wild type. It required a higher light intensity for the saturation of photosynthesis and displayed a greater per chlorophyll light-saturated rate of oxygen evolution than the wild type. The Chl antenna size of the photosystems in the tla4 mutant was only about 65% of that measured in the wild type. Molecular genetic analysis revealed that a single plasmid DNA insertion disrupted two genes on chromosome 11 of the mutant. A complementation study identified the “chloroplast signal recognition particle 54” gene (CpSRP54), as the lesion causing the tla4 phenotype. Disruption of this gene resulted in partial failure to assemble and, therefore, lower levels of light-harvesting Chl-binding proteins in the C. reinhardtii thylakoids. A comparative in silico 3-D structure-modeling analysis revealed that the M-domain of the CpSRP54 of C. reinhardtii possesses a more extended finger loop structure, due to different amino acid composition, as compared to that of the Arabidopsis CpSRP54. The work demonstrated that CpSRP54 deletion in microalgae can serve to generate tla mutants with a markedly smaller photosystem Chl antenna size, improved solar energy conversion efficiency, and photosynthetic productivity in high-density cultures under bright sunlight conditions.

Original languageEnglish
Pages (from-to)45-55
Number of pages11
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1858
Issue number1
DOIs
StatePublished - 2017 Jan 1

Fingerprint

Signal Recognition Particle
Chlamydomonas reinhardtii
Chloroplasts
Chlorophyll
Genes
Antennas
Light
Light-Harvesting Protein Complexes
Chlorophyll Binding Proteins
Solar Energy
Microalgae
Phenotype
Chromosomes, Human, Pair 11
Thylakoids
DNA Transposable Elements
High intensity light
Sunlight
Gene Deletion
Photosynthesis
Protein C

Keywords

  • Chlorophyll antenna size
  • CpSRP54
  • LHC assembly
  • Photosynthesis
  • Productivity
  • TLA technology

Cite this

Jeong, Jooyeon ; Baek, Kwangryul ; Kirst, Henning ; Melis, Anastasios ; Jin, EonSeon. / Loss of CpSRP54 function leads to a truncated light-harvesting antenna size in Chlamydomonas reinhardtii. In: Biochimica et Biophysica Acta - Bioenergetics. 2017 ; Vol. 1858, No. 1. pp. 45-55.
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Loss of CpSRP54 function leads to a truncated light-harvesting antenna size in Chlamydomonas reinhardtii. / Jeong, Jooyeon; Baek, Kwangryul; Kirst, Henning; Melis, Anastasios; Jin, EonSeon.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1858, No. 1, 01.01.2017, p. 45-55.

Research output: Contribution to journalArticle

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T1 - Loss of CpSRP54 function leads to a truncated light-harvesting antenna size in Chlamydomonas reinhardtii

AU - Jeong, Jooyeon

AU - Baek, Kwangryul

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AU - Jin, EonSeon

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