Loss of Calreticulin Uncovers a Critical Role for Calcium in Regulating Cellular Lipid Homeostasis

Wen An Wang, Wen Xin Liu, Serpen Durnaoglu, Sun Kyung Lee, Jihong Lian, Richard Lehner, Joohong Ahnn, Luis B. Agellon, Marek Michalak

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Abstract

A direct link between Ca2+ and lipid homeostasis has not been definitively demonstrated. In this study, we show that manipulation of ER Ca2+ causes the re-distribution of a portion of the intracellular unesterified cholesterol to a pool that is not available to the SCAP-SREBP complex. The SREBP processing pathway in ER Ca2+ depleted cells remained fully functional and responsive to changes in cellular cholesterol status but differed unexpectedly in basal activity. These findings establish the role of Ca2+ in determining the reference set-point for controlling cellular lipid homeostasis. We propose that ER Ca2+ status is an important determinant of the basal sensitivity of the sterol sensing mechanism inherent to the SREBP processing pathway.

Original languageEnglish
Article number5941
JournalScientific reports
Volume7
Issue number1
DOIs
StatePublished - 2017 Dec 1

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Calreticulin
Homeostasis
Cholesterol
Calcium
Lipids
Sterols

Cite this

Wang, W. A., Liu, W. X., Durnaoglu, S., Lee, S. K., Lian, J., Lehner, R., ... Michalak, M. (2017). Loss of Calreticulin Uncovers a Critical Role for Calcium in Regulating Cellular Lipid Homeostasis. Scientific reports, 7(1), [5941]. https://doi.org/10.1038/s41598-017-05734-x
Wang, Wen An ; Liu, Wen Xin ; Durnaoglu, Serpen ; Lee, Sun Kyung ; Lian, Jihong ; Lehner, Richard ; Ahnn, Joohong ; Agellon, Luis B. ; Michalak, Marek. / Loss of Calreticulin Uncovers a Critical Role for Calcium in Regulating Cellular Lipid Homeostasis. In: Scientific reports. 2017 ; Vol. 7, No. 1.
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Wang, WA, Liu, WX, Durnaoglu, S, Lee, SK, Lian, J, Lehner, R, Ahnn, J, Agellon, LB & Michalak, M 2017, 'Loss of Calreticulin Uncovers a Critical Role for Calcium in Regulating Cellular Lipid Homeostasis', Scientific reports, vol. 7, no. 1, 5941. https://doi.org/10.1038/s41598-017-05734-x

Loss of Calreticulin Uncovers a Critical Role for Calcium in Regulating Cellular Lipid Homeostasis. / Wang, Wen An; Liu, Wen Xin; Durnaoglu, Serpen; Lee, Sun Kyung; Lian, Jihong; Lehner, Richard; Ahnn, Joohong; Agellon, Luis B.; Michalak, Marek.

In: Scientific reports, Vol. 7, No. 1, 5941, 01.12.2017.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Wang, Wen An

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