Lobarstin enhances chemosensitivity in human glioblastoma T98G cells

Sojin Kim, Sungsin Jo, Hongki Lee, Tae Ue Kim, Il Chan Kim, Joung Han Yim, Heekyoung Chung

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Background/Aim: Lobarstin is a metabolite occurring from the Antarctic lichen Stereocaulon alpnum. Human glioblastoma is highly resistant to chemotherapy with temozolomide. Lobarstin was examined for its effect on glioblastoma. Materials and Methods: Temozolomide-resistant T98G cells were subjected to toxicity test with temozolomide and/or lobarstin. DNA damage and recovery was assessed by the alkaline comet assay and expression of DNA repair genes was examined by RT-PCR and western blot analysis. Results: Lobarstin alone at 40 μM was toxic against T98G, but had no effect in primary human fibroblasts. Cotreatment of lobarstin with temozolomide yielded enhanced toxicity. Temozolomide-alone or with lobarstin co-treatment gave similar extent of DNA damage. However, the recovery was reduced in co-treated cells. Expression of DNA repair genes, O6-methylguanine-DNA methyltransferase, poly(ADP-ribose) polymerase 1 and ligase 3 were reduced in lobarstin-treated cells. Conclusion: Enhanced sensitivity to temozolomide by lobarstin co-treatment may be attributed to reduced DNA repair.

Original languageEnglish
Pages (from-to)5445-5452
Number of pages8
JournalAnticancer Research
Volume33
Issue number12
StatePublished - 2013 Dec 1

Keywords

  • Chemosensitivity
  • DNA repair
  • Glioblastoma
  • Lobarstin
  • Temozolomide

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    Kim, S., Jo, S., Lee, H., Kim, T. U., Kim, I. C., Yim, J. H., & Chung, H. (2013). Lobarstin enhances chemosensitivity in human glioblastoma T98G cells. Anticancer Research, 33(12), 5445-5452.