Kinetic studies of aspartase from Hafnia alvei by temperature dependence activity changes

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Abstract

The temperature dependence of the kinetic parameters of the aspartase- catalyzed reaction has been examined in the direction of deamination. The pK1 values at 37 °C, 25 °C, 16 °C and 7 °C were 6.2 ± 0.1, 6.3 ± 0.1, 6.7 ± 0.3 and 6.9 ± 0.3, respectively. On the other hand, the pK2 values at 37 °C, 25 °C, 16 °C and 7 °C were 8.1 ± 0.2, 8.3 ± 0.2, 8.2 ± 0.3 and 8.0 ± 0.2, respectively. The enthalpy of ionization, δH(ion), calculated from the slope of pK1, are 6.0 ± 0.3 kcal/mol. These results validate the prediction that aspartase requires a histidine residue for a general base, and a cysteine (or having a carboxyl functional group) for a general acid.

Original languageEnglish
Pages (from-to)379-382
Number of pages4
JournalBulletin of the Korean Chemical Society
Volume21
Issue number4
StatePublished - 2000 Apr 20

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Aspartate Ammonia-Lyase
Thermodynamic properties
Kinetics
Kinetic parameters
Histidine
Functional groups
Ionization
Cysteine
Enthalpy
Temperature
Acids

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title = "Kinetic studies of aspartase from Hafnia alvei by temperature dependence activity changes",
abstract = "The temperature dependence of the kinetic parameters of the aspartase- catalyzed reaction has been examined in the direction of deamination. The pK1 values at 37 °C, 25 °C, 16 °C and 7 °C were 6.2 ± 0.1, 6.3 ± 0.1, 6.7 ± 0.3 and 6.9 ± 0.3, respectively. On the other hand, the pK2 values at 37 °C, 25 °C, 16 °C and 7 °C were 8.1 ± 0.2, 8.3 ± 0.2, 8.2 ± 0.3 and 8.0 ± 0.2, respectively. The enthalpy of ionization, δH(ion), calculated from the slope of pK1, are 6.0 ± 0.3 kcal/mol. These results validate the prediction that aspartase requires a histidine residue for a general base, and a cysteine (or having a carboxyl functional group) for a general acid.",
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Kinetic studies of aspartase from Hafnia alvei by temperature dependence activity changes. / Yoon, Moon-Young.

In: Bulletin of the Korean Chemical Society, Vol. 21, No. 4, 20.04.2000, p. 379-382.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Kinetic studies of aspartase from Hafnia alvei by temperature dependence activity changes

AU - Yoon, Moon-Young

PY - 2000/4/20

Y1 - 2000/4/20

N2 - The temperature dependence of the kinetic parameters of the aspartase- catalyzed reaction has been examined in the direction of deamination. The pK1 values at 37 °C, 25 °C, 16 °C and 7 °C were 6.2 ± 0.1, 6.3 ± 0.1, 6.7 ± 0.3 and 6.9 ± 0.3, respectively. On the other hand, the pK2 values at 37 °C, 25 °C, 16 °C and 7 °C were 8.1 ± 0.2, 8.3 ± 0.2, 8.2 ± 0.3 and 8.0 ± 0.2, respectively. The enthalpy of ionization, δH(ion), calculated from the slope of pK1, are 6.0 ± 0.3 kcal/mol. These results validate the prediction that aspartase requires a histidine residue for a general base, and a cysteine (or having a carboxyl functional group) for a general acid.

AB - The temperature dependence of the kinetic parameters of the aspartase- catalyzed reaction has been examined in the direction of deamination. The pK1 values at 37 °C, 25 °C, 16 °C and 7 °C were 6.2 ± 0.1, 6.3 ± 0.1, 6.7 ± 0.3 and 6.9 ± 0.3, respectively. On the other hand, the pK2 values at 37 °C, 25 °C, 16 °C and 7 °C were 8.1 ± 0.2, 8.3 ± 0.2, 8.2 ± 0.3 and 8.0 ± 0.2, respectively. The enthalpy of ionization, δH(ion), calculated from the slope of pK1, are 6.0 ± 0.3 kcal/mol. These results validate the prediction that aspartase requires a histidine residue for a general base, and a cysteine (or having a carboxyl functional group) for a general acid.

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