Involvement of MAPK activation in chemokine or COX-2 productions by Toxoplasma gondii.

Ji Young Kim, Myoung Hee Ahn, Hyun Ouk Song, Jong Hak Choi, Jae-Sook Ryu, Duk Young Min, Myung Hwan Cho

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This experiment focused on MAPK activation in host cell invasion and replication of T. gondii, as well as the expression of CC chemokines, MCP-1 and MIP-1 alpha , and enzyme, COX-2/prostaglandin E2 (PGE2) in infected cells via western blot, [3H]-uracil incorporation assay, ELISA and RT-PCR. The phosphorylation of ERK1/2 and p38 in infected HeLa cells was detected at 1 hr and/or 6 hr postinfection (PI). Tachyzoite proliferation was reduced by p38 or JNK MAPK inhibitors. MCP-1 secretion was enhanced in infected peritoneal macrophages at 6 hr PI. MIP-1 alpha mRNA was increased in macrophages at 18 hr PI. MCP-1 and MIP-1 alpha were reduced after treatment with inhibitors of ERK1/2 and JNK MAPKs. COX-2 mRNA gradually increased in infected RAW 264.7 cells and the secretion of COX-2 peaked at 6 hr PI. The inhibitor of JNK suppressed COX-2 expression. PGE2 from infected RAW 264.7 cells was increased and synthesis was suppressed by PD98059, SB203580, and SP600125. In this study, the activation of p38, JNK and/or ERK1/2 MAPKs occurred during the invasion and proliferation of T. gondii tachyzoites in HeLa cells. Also, increased secretion and expression of MCP-1, MIP-1 alpha , COX-2 and PGE2 were detected in infected macrophages, and appeared to occur via MAPK signaling pathways.

Original languageEnglish
Pages (from-to)197-207
Number of pages11
JournalThe Korean journal of parasitology
Volume44
Issue number3
DOIs
StatePublished - 2006 Jan 1

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Toxoplasma
Chemokines
Dinoprostone
HeLa Cells
Macrophages
CC Chemokines
Messenger RNA
Uracil
Peritoneal Macrophages
Western Blotting
Enzyme-Linked Immunosorbent Assay
Phosphorylation
Polymerase Chain Reaction
Enzymes
RAW 264.7 Cells
Therapeutics

Cite this

Kim, Ji Young ; Ahn, Myoung Hee ; Song, Hyun Ouk ; Choi, Jong Hak ; Ryu, Jae-Sook ; Min, Duk Young ; Cho, Myung Hwan. / Involvement of MAPK activation in chemokine or COX-2 productions by Toxoplasma gondii. In: The Korean journal of parasitology. 2006 ; Vol. 44, No. 3. pp. 197-207.
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abstract = "This experiment focused on MAPK activation in host cell invasion and replication of T. gondii, as well as the expression of CC chemokines, MCP-1 and MIP-1 alpha , and enzyme, COX-2/prostaglandin E2 (PGE2) in infected cells via western blot, [3H]-uracil incorporation assay, ELISA and RT-PCR. The phosphorylation of ERK1/2 and p38 in infected HeLa cells was detected at 1 hr and/or 6 hr postinfection (PI). Tachyzoite proliferation was reduced by p38 or JNK MAPK inhibitors. MCP-1 secretion was enhanced in infected peritoneal macrophages at 6 hr PI. MIP-1 alpha mRNA was increased in macrophages at 18 hr PI. MCP-1 and MIP-1 alpha were reduced after treatment with inhibitors of ERK1/2 and JNK MAPKs. COX-2 mRNA gradually increased in infected RAW 264.7 cells and the secretion of COX-2 peaked at 6 hr PI. The inhibitor of JNK suppressed COX-2 expression. PGE2 from infected RAW 264.7 cells was increased and synthesis was suppressed by PD98059, SB203580, and SP600125. In this study, the activation of p38, JNK and/or ERK1/2 MAPKs occurred during the invasion and proliferation of T. gondii tachyzoites in HeLa cells. Also, increased secretion and expression of MCP-1, MIP-1 alpha , COX-2 and PGE2 were detected in infected macrophages, and appeared to occur via MAPK signaling pathways.",
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Involvement of MAPK activation in chemokine or COX-2 productions by Toxoplasma gondii. / Kim, Ji Young; Ahn, Myoung Hee; Song, Hyun Ouk; Choi, Jong Hak; Ryu, Jae-Sook; Min, Duk Young; Cho, Myung Hwan.

In: The Korean journal of parasitology, Vol. 44, No. 3, 01.01.2006, p. 197-207.

Research output: Contribution to journalArticle

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AU - Kim, Ji Young

AU - Ahn, Myoung Hee

AU - Song, Hyun Ouk

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