Interdigitated microelectrode based impedance biosensor for detection of salmonella enteritidis in food samples

G. Kim, M. Morgan, B. K. Hahm, A. Bhunia, J. H. Mun, A. S. Om

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Salmonella enteritidis outbreaks continue to occur, and S. enteritidis-related outbreaks from various food sources have increased public awareness of this pathogen. Conventional methods for pathogens detection and identification are labor-intensive and take days to complete. Some immunological rapid assays are developed, but these assays still require prolonged enrichment steps. Recently developed biosensors have shown great potential for the rapid detection of foodborne pathogens. To develop the biosensor, an interdigitated microelectrode (IME) was fabricated by using semiconductor fabrication process. Anti- Salmonella antibodies were immobilized based on avidin-biotin binding on the surface of the IME to form an active sensing layer. To increase the sensitivity of the sensor, three types of sensors that have different electrode gap sizes (2 μm, 5 μm, 10 μm) were fabricated and tested. The impedimetric biosensor could detect 103 CFU/mL of Salmonella in pork meat extract with an incubation time of 5 minutes. This method may provide a simple, rapid and sensitive method to detect foodborne pathogens.

Original languageEnglish
Article number052044
JournalJournal of Physics: Conference Series
Volume100
Issue numberPART 5
DOIs
StatePublished - 2008 Mar 1

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salmonella
pathogens
bioinstrumentation
food
impedance
biotin
labor
sensors
antibodies
fabrication
electrodes
sensitivity

Cite this

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title = "Interdigitated microelectrode based impedance biosensor for detection of salmonella enteritidis in food samples",
abstract = "Salmonella enteritidis outbreaks continue to occur, and S. enteritidis-related outbreaks from various food sources have increased public awareness of this pathogen. Conventional methods for pathogens detection and identification are labor-intensive and take days to complete. Some immunological rapid assays are developed, but these assays still require prolonged enrichment steps. Recently developed biosensors have shown great potential for the rapid detection of foodborne pathogens. To develop the biosensor, an interdigitated microelectrode (IME) was fabricated by using semiconductor fabrication process. Anti- Salmonella antibodies were immobilized based on avidin-biotin binding on the surface of the IME to form an active sensing layer. To increase the sensitivity of the sensor, three types of sensors that have different electrode gap sizes (2 μm, 5 μm, 10 μm) were fabricated and tested. The impedimetric biosensor could detect 103 CFU/mL of Salmonella in pork meat extract with an incubation time of 5 minutes. This method may provide a simple, rapid and sensitive method to detect foodborne pathogens.",
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Interdigitated microelectrode based impedance biosensor for detection of salmonella enteritidis in food samples. / Kim, G.; Morgan, M.; Hahm, B. K.; Bhunia, A.; Mun, J. H.; Om, A. S.

In: Journal of Physics: Conference Series, Vol. 100, No. PART 5, 052044, 01.03.2008.

Research output: Contribution to journalArticle

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AU - Morgan, M.

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AU - Mun, J. H.

AU - Om, A. S.

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