Inhibition of human term placental and fetal liver glutathione-S-transferases by fatty acids and fatty acid esters

Ashoke Mitra, Sanjay Govindwar, Pius Joseph, Arun Kulkarni

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Glutathione-S-transferase (GST) activity from human term placenta and human fetal liver towards 1 -chloro-2,4-dinitrobenzene as the second substrate was significantly inhibited by the saturated fatty acids, stearic (SA) and palmitic (PA) acids and fatty acid esters, ascorbyl stearate (Asc-S) and ascorbyl palmitate (Asc-P). The nature of inhibition of human placental GST was competitive towards CDNB with Ki values of 3.1, 10.0, 13.5 and 18.5 μM for Asc-S, Asc-P, PA and SA, respectively. The inhibitory effect of Asc-S on human term placental GST was reversible. I50 values for Asc-S, Asc-P, SA and PA were 15, 45, 83 and 78 μM, respectively, for partially purified human fetal liver GSTs and 21, 6, 88 and 117 μM. respectively, for partially pure rat liver GSTs. The evidence suggests that Asc-S, Asc-P, SA and PA are potent inhibitors especially of the pi-class of GST.

Original languageEnglish
Pages (from-to)281-288
Number of pages8
JournalToxicology Letters
Volume60
Issue number3
DOIs
StatePublished - 1992 May
Externally publishedYes

Keywords

  • Ascorbyl esters
  • Chemotherapeutic drug resistance
  • Glutathione-S-transferase, pi-class
  • Glutathione-S-transferases
  • Inhibition

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