Induction of apoptosis by all-trans-retinoic acid and C2-ceramide treatment in rat stromal-vascular cultures

H. S. Kim, D. B. Hausman, M. M. Compton, R. G. Dean, R. J. Martin, G. J. Hausman, D. L. Hartzell, C. A. Baile

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Apoptosis of preadipocytes and adipocytes contributes to the balance of adipose tissue mass by reducing adipocyte number. To address this phenomenon, we treated cultured rat S-V cells with all-trans-retinoic acid (RA) (10 μM) or C2-ceramide (50 μM) during adipogenesis. Gel electrophoresis of DNA from treated cells cultured in serum-free medium showed that 10 μM RA or 50 μM ceramide induced a distinct laddering pattern of DNA fragments. Cellular caspase 3 activity, another marker of apoptosis, was increased by RA (10 μM) (P < 0.05), but not by 50 μm C2-ceramide. RT-PCR results showed that RA (10 μM) decreased the expression of Bcl-2 mRNA. These results suggest that fat cell loss by apoptosis can be regulated, in part, by RA (10 μM) which increases caspase 3 activity and decreases Bcl-2 expression in rat S-V cells. C2-ceramide apparently works through a different cellular mechanism to induce apoptosis. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)76-80
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume270
Issue number1
DOIs
StatePublished - 2000 Apr 2

Keywords

  • All-trans retinoic acid
  • Apoptosis
  • C2-ceramide
  • Rat stromal-vascular (S-V) cells

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