In vitro generation of mature dopamine neurons by decreasing and delaying the expression of exogenous Nurr1

Chang Hwan Park, Mi Sun Lim, Yong Hee Rhee, Sang Hoon Yi, Boe Kyoung Kim, Jae Won Shim, Yong Ho Kim, Sung Jun Jung, Sang Hun Lee

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Neural stem/progenitor cell (NSC/NPC) cultures can be a source of dopamine (DA) neurons for experimental and transplantation purposes. Nurr1, a steroid receptor transcription factor, can overcome the limitations associated with differentiation of cultured NPCs into DA neurons. However, forced Nurr1 expression in NPC cultures generates non-neuronal and/or immature DA cells. We show here that the Nurr1 level and period of expression crucially affect the differentiation and maturation of Nurr1-induced DA neurons. Mature DA neurons were generated by manipulating Nurr1 expression patterns to resemble those in the developing midbrain.

Original languageEnglish
Pages (from-to)2447-2451
Number of pages5
JournalDevelopment (Cambridge)
Volume139
Issue number13
DOIs
StatePublished - 2012 Jul 1

Keywords

  • Dopamine neurons
  • Doxycycline inducible expression system
  • Neuronal maturation
  • Nurr1
  • Parkinson's disease

Fingerprint Dive into the research topics of 'In vitro generation of mature dopamine neurons by decreasing and delaying the expression of exogenous Nurr1'. Together they form a unique fingerprint.

  • Cite this